MABC544
抗 PLK-4 抗体，克隆 6H5
clone 6H5, from mouse
别名:
Serine/threonine-protein kinase PLK4, Polo-like kinase 4, PLK-4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak
产品名称
抗 PLK-4 抗体，克隆 6H5, clone 6H5, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6H5, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG3κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PLK4(10733)
Analysis Note
对照
U2Os细胞裂解液
U2Os细胞裂解液
通过蛋白质印迹法在 U2Os 细胞裂解物中进行评价。
蛋白质印迹分析：4.0 µg/mL 该抗体在 10 µg HCT116 细胞裂解物中检测到 U2Os。
蛋白质印迹分析：4.0 µg/mL 该抗体在 10 µg HCT116 细胞裂解物中检测到 U2Os。
Application
使用这种小鼠单克隆抗体，抗PLK-4 抗体，克隆 6H5，检测 Polo 样激酶 (PLK)，该抗体经过验证可用于蛋白质印迹、ICC & IP。
免疫细胞化学分析：代表性批次在用对照和 Cep152 siRNA 转染的 U20S 细胞裂解物中检测到 PLK-4，但在用 PLK4 siRNA 转染的 U2OS 细胞裂解物中未检测到。(Cizmecioglu, O. et al. (2010).J Cell Biol. 191(4):731-739).
免疫沉淀分析：一个代表性批次在共转染了带有 Flag 标签的 Gcp6 和带有 Myc 标签的 Plk-4 的 HeLa 细胞裂解物中免疫沉淀了 PLK-4(Bahtz, R. et al. (2012)。J Cell Sci. 125 (Pt 2): 486-496).
免疫沉淀分析：一个代表性批次在共转染了带有 Flag 标签的 Gcp6 和带有 Myc 标签的 Plk-4 的 HeLa 细胞裂解物中免疫沉淀了 PLK-4(Bahtz, R. et al. (2012)。J Cell Sci. 125 (Pt 2): 486-496).
研究子类别
凋亡-附加
凋亡-附加
研究类别
细胞凋亡 & 癌症
细胞凋亡 & 癌症
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
丝氨酸/苏氨酸蛋白激酶 PLK4 (PLK-4) 是蛋白激酶超家族的成员，在中心粒复制中发挥重要作用。PLK-4 触发亲本中心粒圆柱表面上的原中心粒形成，导致中心粒生物发生蛋白的募集。它可能参与肿瘤发生，因为在肿瘤中经常观察到中心体畸变。PLK-4 还可能通过磷酸化 HAND1 参与滋养层分化，导致 HAND1 和 MDFIC 之间的破坏，然后激活 HAND1。
可观察到109 kDa的条带
Immunogen
对应于人 PLK-4 的线性肽。
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
纯化的小鼠单克隆IgG3κ，溶于含0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05%叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Midori Ohta et al.
Cell reports, 23(11), 3160-3169 (2018-06-14)
The number of centrioles is tightly controlled to ensure bipolar spindle assembly, which is a prerequisite to maintain genome integrity. However, our understanding of the fundamental principle that governs the formation of a single procentriole per parental centriole is incomplete.
Dasom Gwon et al.
Molecules and cells, 42(12), 840-849 (2019-11-15)
The spatiotemporal mitotic processes are controlled qualitatively by phosphorylation and qualitatively by ubiquitination. Although the SKP1-CUL1-F-box protein (SCF) complex and the anaphase-promoting complex/cyclosome (APC/C) mainly mediate ubiquitin-dependent proteolysis of mitotic regulators, the E3 ligase for a large portion of mitotic
Midori Ohta et al.
Nature communications, 5, 5267-5267 (2014-10-25)
Formation of one procentriole next to each pre-existing centriole is essential for centrosome duplication, robust bipolar spindle assembly and maintenance of genome integrity. However, the mechanisms maintaining strict control over centriole copy number are incompletely understood. Here we show that
Onur Rojhat Karasu et al.
The Journal of cell biology, 221(12) (2022-11-01)
The centriole is the microtubule-based backbone that ensures integrity, function, and cell cycle-dependent duplication of centrosomes. Mostly unclear mechanisms control structural integrity of centrioles. Here, we show that the centrosome protein CEP350 functions as scaffold that coordinates distal-end properties of
Satoko Yoshiba et al.
Journal of cell science, 132(12) (2019-06-06)
At the onset of procentriole formation, a structure called the cartwheel is formed adjacent to the pre-existing centriole. SAS-6 proteins are thought to constitute the hub of the cartwheel structure. However, the exact function of the cartwheel in the process
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