biological source
rat
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ABL-93, monoclonal
species reactivity
mouse
technique(s)
immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Lamp2(16784)
General description
溶酶体相关膜蛋白2(LAMP-2)是溶酶体相关膜蛋白家族的一种高度糖苷化蛋白。这组蛋白构成溶酶体膜的重要部分,是通过与碳水化合物受体相互作用形成细胞粘附的重要介质。LAMP-2是溶酶体膜的重要组成部分,在溶酶体生物起源中起重要作用。 它似乎也参与吞噬和自噬的后期阶段,可保护溶酶体膜不被消化、协助信号转导、在溶酶体环境中调节酸度水平,并参与粘附。LAMP-2表达突变与溶酶体贮积病(LSD)以及Danon病(一种X连锁疾病,以骨骼肌病、智力迟钝和肥厚性心肌病为特征)相关。
观察到的分子量~ 80 kDa。计算出的分子量为45 kDa, LAMP-2 被高度糖苷化,根据发表的数据,通常在80-120 kDa分子量范围内观察到(Chen, 1985; Ferguson, 2009)。
Immunogen
从NIH/3T3细胞纯化的糖蛋白,对应于小鼠LAMP-2。
Application
免疫沉淀分析: 该抗体已被证明可在HaNIH细胞中对LAMP-2进行免疫沉淀(Chen, 1985)。
免疫荧光分析: 该抗体已被证明可以检测小鼠大脑中的LAMP-2(Ferguson, 2009)。
免疫荧光分析: 该抗体已被证明可以检测小鼠大脑中的LAMP-2(Ferguson, 2009)。
抗-LAMP-2抗体,克隆ABL-93是用于WB、IP & IF的抗LAMP-2抗体。
研究子类别
凋亡-附加
凋亡-附加
研究类别
细胞凋亡 & 癌症
细胞凋亡 & 癌症
Biochem/physiol Actions
该抗体可识别LAMP-2。
Physical form
形式:纯化
纯化的大鼠单克隆IgG2aκ溶于含0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl和0.05%叠氮化钠的缓冲液中。
蛋白G
Preparation Note
自收到之日起,在2-8°C条件下可稳定保存1年。
Analysis Note
对照
NIH/3T3细胞裂解液
NIH/3T3细胞裂解液
通过蛋白质印迹在NIH/3T3细胞裂解液中进行了评估。
蛋白质印迹分析:1 µg/mL的该抗体在10µg NIH/3T3细胞裂解物中检测到LAMP-2。
蛋白质印迹分析:1 µg/mL的该抗体在10µg NIH/3T3细胞裂解物中检测到LAMP-2。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Disclaimer
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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