MABC202
Anti-APC Antibody, clone FE9
clone FE9, from mouse
别名:
Adenomatous polyposis coli protein, Protein APC, Deleted in polyposis 2.5
产品名称
Anti-APC Antibody, clone FE9, clone FE9, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FE9, monoclonal
species reactivity
human
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... APC(324)
Analysis Note
Evaluated by Western Blot in SW480 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected APC in 10 µg of SW480 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected APC in 10 µg of SW480 cell lysate.
Application
Anti-APC Antibody, clone FE9 is an antibody against APC for use in Western Blotting.
Biochem/physiol Actions
This antibody recognizes the N-terminus of APC.
General description
Adenomatous polyposis coli protein (APC) is a ubiquitous multidomain protein that has a well documented role as a tumor suppressor. The mechanism of APC-mediated tumor suppression is still an area of active investigation; however, several studies suggest that APC is a negative regulator of the Wnt signaling pathway as it downregulates β-catenin via interactions with Axin/GSK3-β complex, thereby preventing transcription of genes such as MYC that contribute to cell proliferation. Defective APC proteins contribute to the initiation and proliferation of various types of cancers, including familial adenomatous polyposis. However, other studies have shown that APC interacts with a range of other proteins such as the EB1 microtubule-binding proteins, to regulate other processes such as cell migration.
~147 kDa observed. Uniprot describes the full length protein at ~312 kDa This antibody was shown to detect the truncated form at ~147 kDa
Immunogen
Linear peptide corresponding to the N-terminus of human APC.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Inducible in vivo genome editing with CRISPR-Cas9.
Dow, LE; Fisher, J; O'Rourke, KP; Muley, A; Kastenhuber, ER; Livshits, G; Tschaharganeh et al.
Nature Biotechnology null
Weiran Feng et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(32) (2021-08-07)
The increasing complexity of different cell types revealed by single-cell analysis of tissues presents challenges in efficiently elucidating their functions. Here we show, using prostate as a model tissue, that primary organoids and freshly isolated epithelial cells can be CRISPR
Thai Q Tran et al.
Nature cancer, 1(3), 345-358 (2020-08-25)
Genetic-driven deregulation of the Wnt pathway is crucial but not sufficient for colorectal cancer (CRC) tumourigenesis. Here, we show that environmental glutamine restriction further augments Wnt signaling in APC mutant intestinal organoids to promote stemness and leads to adenocarcinoma formation
Maria Paz Zafra et al.
Nature biotechnology, 36(9), 888-893 (2018-07-04)
CRISPR base editing enables the creation of targeted single-base conversions without generating double-stranded breaks. However, the efficiency of current base editors is very low in many cell types. We reengineered the sequences of BE3, BE4Gam, and xBE3 by codon optimization
Bo Cen et al.
Oncogene, 40(41), 5984-5992 (2021-08-14)
PD-L1 expression is elevated in various human cancers, including colorectal cancer. High levels of PD-L1 expressed on tumor epithelial cells are one of the potential mechanisms by which tumor cells become resistant to immune attack. However, PD-L1 regulation in tumor
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