Anti-Apaf-1 Antibody, clone 18H2

Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.

Clone 18H2 detects Apaf-1 in human and mouse cells. It targets an epitope within the first 97 amino acids from the N-terminal region containing the CARD domain.

Epitope: N-terminus

Recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine Apaf-1.

Anti-Apaf-1 Antibody, clone 18H2, Cat. No. MABC1187, is a rat monoclonal antibody that specifically detects Apaf-1. It has been tested for use in Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Scott, C.L., et. al. (2004). J Cell Biol. 164(1):89-96).

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Marsden, V.S., et. al. (2006). Blood. 107(5):1872-7).

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apaf-1 in 10 mg of SH-SY5Y cell lysate.

~130 kDa observed;141 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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