Anti-PME1 Antibody, clone 8A6-F8

Protein phosphatase methylesterase 1 (UniProt: Q8BVQ5; also known as PME1; EC:3.1.1.89) is encoded by the Ppme1 (also known as Pme1) gene (Gene ID: 72590) in murine species. PME1 is a member of the AB hydrolase family that shows ubiquitous distribution, but is highly expressed in testis and brain. PME-1 is shown to be PMSF-resistant and okadaic acid-sensitive methyltransferase. PME-1 contains a motif found in lipases that utilize a catalytic triad-activated serine as their active site nucleophile, and has other scattered homology with other lipases in which this motif is conserved. PME1 catalyzes the demethylation of proteins that have been reversibly carboxymethylated. PME1 is shown to demethylate and inactivate protein phosphatase 2A (PP2A). Its binding to the catalytic site of PP2A displaces manganese ion that results in the inactivation of PP2A. Targeted disruption of the Ppme1 gene is shown to cause perinatal lethality in mice, resulting from a virtually complete loss of the demethylated form of PP2A in the nervous system and peripheral tissues.

Clone 8A6-F8 specifically stains PME-1 in paraffin sections of human pancreas, testis, and cerebral cortex.

His-tagged full length recombinant mouse Protein phosphatase methylesterase 1.

Detect Protein phosphatase methylesterase 1 using this mouse monoclonal Anti-PME1 Antibody, clone 8A6-F8, Cat. No. MABC1183. It is used in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PME1 in human testis and human cerebral cortex tissue.

Immunoprecipitation Analysis: A representative lot detected PME1 in NIH3T3 mouse fibroblasts, wild-type, but not in PME1 knock-out mouse embryonic fibroblasts. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).

Western Blotting Analysis: A 1:50 dilution from a representative lot detected PME1 in HeLa, NIH3T3, Rat1, BHK21, and CV-1 lysates. (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).

Research Category
Apoptosis & Cancer

Evaluated by Immunohistochemistry in human pancreas tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected PME1 in human pancreas tissue.

42.25 kDa calculated.

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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