Anti-HAF/SART1 Antibody, clone 1-2

U4/U6.U5 tri-snRNP-associated protein 1 (UniProt O43290; also known as Allergen Hom s 1, HAF, hSART-1, hSnu66, Hypoxia-associated factor, SART-1, SNU66 homolog, Squamous cell carcinoma antigen recognized by T-cells 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein) is encoded by the SART1 (also known as HAF) gene (Gene ID 9092) in human. The hypoxia-associated factor (HAF) is an E3 ubiquitin ligase that mediates the ubiquitination and degradation of hypoxia-inducible factor 1α (HIF-1α). HAF also binds to HIF-2α and promotes HIF-2 transactivation instead of promoting HIF-2α degradation. In addition, hypoxia-dependent HAF SUMOylation enhances HAF interaction with HIF-2α and induction of HIF-2 target genes transcription, such as OCT-3/4, SOX2 and NANOG. HAF therefore promotes stem cell characteristics, aggressive tumor growth and invasion by switching cells from HIF-1α- to HIF-2α-dependent signaling. HAF overexpression enhances glioblastoma cells that express both HIF-1α and HIF-2α to initiate tumors as intracranial xenografts in mice. On the other hand, HAF overexpression results in decreased xenograft tumor growth of HT29 colon carcinoma cells that only express HIF-1α.

Clone 1-2 detected HAF shRNA-mediated HAF knockdown in MIA PaCa-2 human pancreatic carcinoma cells.

GST-tagged recombinant human HAF/SART1 C-terminal fragment.

Detect HAF/SART1 using this mouse monoclonal Anti-HAF/SART1, clone 1-2 Antibody, Cat. No. MABC1149, validated for use in Western Blotting and Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected HAF/SART1 in A431 cells.

Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected HAF/SART1 in 10 µg of Jurkat cell lysate.

Immunocytochemistry Analysis: A representative lot detected a reduced HAF immunoreactivity in MIA PaCa-2 human pancreatic carcinoma cells following HAF shRNA treatment (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunocytochemistry Analysis: A representative lot detected HAF in HeLa and PANC-1 cells (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunocytochemistry Analysis: A representative lot detected an increased HAF immunoreactivity in 786-O human kidney cancer cells transfected to overexpress HAF (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Immunohistochemistry Analysis: A representative lot detected HAF immunoreactivity in 786-O xenograft (Courtesy of Mei Yee Koh, Ph.D., Sanford-Burnham Medical Research Institute, La Jolla, CA USA).

Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected HAF/SART1 in 10 µg of HeLa cell lysate.

~110 kDa observed. 90.26 kDa calculated. The larger-than-calculated target band size is consistent with that reported in the literature (Koh, M.Y. et al. (2015). Cancer Res. 75(2):316-329). Uncharacterized bands may be observed in some lysate(s).

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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