Anti-CIP2A Antibody, clone 6G8-G1

Protein CIP2A (UniProt: Q8TCG1; also known as CIP2A, Cancerous inhibitor of PP2A, p90 autoantigen) is encoded by the KIAA1524 (also known as CIP2A) gene (Gene ID: 57650) in human. CIP2A is a single pass membrane protein that exhibits dynamic changes in localization, including the cytoplasm and centrosome, depending on the stage of the cell cycle. It is expressed at low levels in most tissues, however, it is overexpressed in several cancers, including head-and-neck squamous cell carcinomas and liver cancer cells. Levels of CIP2A are correlated with the proliferation of human cancer cells and with cancer progression. It acts as an oncoprotein and inhibits protein phosphatase 2A activity and thereby stabilizes c-Myc in human malignancies. It promotes anchorage-independent cell growth and tumor formation. Higher activity of c-Myc rapidly induces the activation of cyclin E-cdk2 kinase activity. Concurrently, the release of p27Kip1 from Cdk2 complexes enhances cell proliferation. In CIP2A depleted cells, centrosome separation and the mitotic spindle dynamics were impaired, which leads to the activation of spindle assembly checkpoint signaling and, ultimately, delayed cell division time. CIP2A is also reported to interact with NIMA (never in mitosis gene A)-related kinase 2 (NEK2) during G2/M phase and enhances NEK2 kinase activity that facilitates centrosome separation in a PP1- and PP2A-independent manner. (Ref.: Jeong, AL et al. (2014). J. Biol. Chem. 289(1): 28-40).

Clone 6G8-G1 specifically detects CIP2A in HEK293 cells overexpressing CIP2A.

Epitope: N-terminus

His-tagged recombinant fragment from the N-terminal region of human CIP2A.

Detect CIP2A using this mouse monoclonal Anti-CIP2A Antibody, clone 6G8-G1, Cat. No. MABC1147, tested in Western Blotting.

Research Category
Apoptosis & Cancer

Evaluated by Western Blotting in HEK293 cells overexpressing CIP2A.

Western Blotting Analysis: 1 µg/mL of this antibody detected CIP2A in 10 µg of lysate from HEK293 cells overexpressing CIP2A.

~102 kDa observed; 102.18 calculated. Uncharacterized bands may be observed in some lysate(s).

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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