Anti-TIMP-4 Antibody, clone 9:4-7

Tissue inhibitor of metalloproteinases 4 (UniProt: Q99727; also known as Metalloproteinase inhibitor 4, TIMP-4) is encoded by the TIMP4 gene (Gene ID: 7079) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins which are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMP-4 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has two MMP-binding regions (aa 30-33 and 99-100). TIMP-4 expressed is observed in heart, brain, pancreas, kidney, colon, testes, and adipose tissue. However, elevated levels of TIMP-4 have been reported in various cancers tissues, including breast, ovary, prostate, colon and kidney and are associated with poor survival prognosis. Hence, it can serve as a useful marker for disease progression. TIMP-4 is shown to complex MMPs and irreversibly inactivates them by binding to their catalytic zinc cofactor. This clone (9:4 7) is specific for human TIMP-4 and does not exhibit any cross-reactivity to other TIMPs. (Ref.: Gomez, DE., et al. (1997). Eur. J. Cell. Biol. 74(2); 111-122; Donover, PS et AL (2010). J. Cell. Biochem. 110(5); 1255-1261).

Clone 9:4-7 specifically detects human TIMP-4.

GST-tagged full-length recombinant human TIMP-4.

Anti-TIMP-4, clone 9:4-7, Cat. No. MABC1142, is a mouse monoclonal antibody that detects Tissue Inhibitor of Matrix Metalloproteinase 4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected TIMP-4 in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected TIMP-4 in Immunohistochemistry applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

ELISA Analysis: A representative lot detected TIMP-4 in ELISA applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

Western Blotting Analysis: A representative lot detected TIMP-4 in Western Blotting applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

Research Category
Apoptosis & Cancer

Evaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected TIMP-4 in human breast cancer tissue sections.

25.50 kDa calculated.

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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