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生物来源
mouse
质量水平
偶联物
FITC conjugate
抗体形式
ascites fluid
克隆
GC270, monoclonal
制造商/商品名称
Chemicon®
技术
flow cytometry: suitable
immunofluorescence: suitable
同位素/亚型
IgG2a
运输
wet ice
靶向翻译后修饰
unmodified
特异性
This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.
免疫原
Protein I preparation from Neisseria gonorrhoeae.
应用
Fluorescein conjugated mouse IgG2a is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.
SUGGESTED USAGE
Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
SUGGESTED USAGE
Flow cytometry and immunofluorescence - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl buffer.
Mouse IgG2a Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Research Category
Secondary & Control Antibodies
Epitope Tags & General Use
Secondary & Control Antibodies
Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies
Isotype Control Antibodies
外形
Purified from mouse ascitic fluid. Fluorescein conjugated, ready to use. The antibody is supplied in 1.0ml phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
储存及稳定性
Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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