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生物来源
mouse
质量水平
抗体形式
ascites fluid
克隆
5A8.2, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
运输
wet ice
特异性
Reacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
With CMV the antigens expressed at different times are listed as:
Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.
Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.
Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
免疫原
Affinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Epitope: early
应用
Anti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.
Immunochemistry.
IFA at 1:3,000-1:6,000 on acetone fixed cells.
Does not work with paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
IFA at 1:3,000-1:6,000 on acetone fixed cells.
Does not work with paraffin embedded tissue sections.
Dilute with buffer pH 7.4-7.6 to desired working volume.
For extensive dilution, protein containing or other stabilizing medium should be used.
Final working dilutions must be determined by end user.
外形
Format: Purified
Purified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Yi-Chieh Perng et al.
Journal of virology, 85(10), 4841-4852 (2011-03-04)
In this study, we adopted a conditional protein genetic approach to characterize the role of the human cytomegalovirus (HCMV) gene UL79 during virus infection. We constructed ADddUL79, a recombinant HCMV in which the annotated UL79 open reading frame (ORF) was
Christopher B Ball et al.
mBio, 13(3), e0033722-e0033722 (2022-05-18)
Human cytomegalovirus (HCMV) immediate-early 2 (IE2) protein is a multifunctional transcription factor that is essential for lytic HCMV infection. IE2 functions as an activator of viral early genes, negatively regulates its own promoter, and is required for viral replication. The
Internal deletions of IE2 86 and loss of the late IE2 60 and IE2 40 proteins encoded by human cytomegalovirus affect the levels of UL84 protein but not the amount of UL84 mRNA or the loading and distribution of the mRNA on polysomes.
Sanders, Rebecca L, et al.
Journal of virology, 82, 11383-11397 (2008)
Blair L Strang et al.
PloS one, 13(7), e0201321-e0201321 (2018-07-27)
Chemogenomic approaches involving highly annotated compound sets and cell based high throughput screening are emerging as a means to identify novel drug targets. We have previously screened a collection of highly characterized kinase inhibitors (Khan et al., Journal of General
Development of cell lines that provide tightly controlled temporal translation of the human cytomegalovirus IE2 proteins for complementation and functional analyses of growth-impaired and nonviable IE2 mutant viruses.
Sanders, RL; Clark, CL; Morello, CS; Spector, DH
Journal of virology null
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