MAB5448
Anti-Neurofilament H (200 kDa) Antibody
CHEMICON®, rat monoclonal, TA51
别名:
NF-H
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关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
TA51, monoclonal
Species reactivity:
human, rat, hamster, monkey
Application:
ELISA
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Technique(s):
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
18
Uniprot accession no.:
产品名称
抗-神经丝NF抗体，克隆TA51, culture supernatant, clone TA51, Chemicon®
biological source
rat
antibody form
culture supernatant
antibody product type
primary antibodies
clone
TA51, monoclonal
species reactivity
human, rat, hamster, monkey
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NEFH(4744)
rat ... Nefh(24587)
rhesus monkey ... Nefh(717705)
相关类别
Application
抗神经丝NF-H抗体（克隆TA51 ）是一种针对神经丝NF-H的抗体，用于 ELISA、IP、WB、IH。
研究子类别
神经丝&神经元代谢
神经&胶质标记物
神经丝&神经元代谢
神经&胶质标记物
研究类别
神经科学
神经科学
蛋白质印迹：在还原样品中鉴定出~ 200kDa的条带。使用Tris基缓冲液以得到最佳结果，因为该表位是磷酸盐依赖性的，CNS的不同区域被立即切开，并以i ml/100 mg湿组织的比率在BUST 缓冲液（8 M 脲、0.5% SDS、2% 13-巯基乙醇、50 mM Tris，pH 7.4）中均质。将组织悬浮液超声处理并在TL-100超离心机（Beckman Instruments, Fullerton,加州）中在25°C、100,000 g 下离心
1小时，并测定上清液中的蛋白浓度。蛋白在7.5% SDS-PAGE 凝胶上分离（Tu.P et al (1995) J Cell Biol 129:1629-1640）
冷冻切片的免疫组化；原始样品在石蜡组织中无效{Carden et al, 1987}。
最佳工作稀释度必须由最终用户进行确定。
1小时，并测定上清液中的蛋白浓度。蛋白在7.5% SDS-PAGE 凝胶上分离（Tu.P et al (1995) J Cell Biol 129:1629-1640）
冷冻切片的免疫组化；原始样品在石蜡组织中无效{Carden et al, 1987}。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
神经丝H（NF-H），羧基末端（磷酸盐依赖性(+)）。克隆对磷酸化 NF-H有特异性（Carden et al (1987) 7(11):3489）。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
表位：羧基末端磷酸化依赖性的，位于NFH中多磷酸化重复序列内部。
Physical form
来自灌注系统的未纯化组织培养物上清液，在装入小瓶之前通过0.2μ膜过滤。产品包含20%FBS和环丙沙星，终浓度为10μg/mL。
Preparation Note
自收到之日起，在 -20°C 以未稀释的等分试样可保存 12 个月。应避免反复冻/融循环。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Jean Defourny et al.
Nature communications, 4, 1438-1438 (2013-02-07)
Hearing requires an optimal afferent innervation of sensory hair cells by spiral ganglion neurons in the cochlea. Here we report that complementary expression of ephrin-A5 in hair cells and EphA4 receptor among spiral ganglion neuron populations controls the targeting of
Natasha Sukhanov et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(7), 1393-1400 (2021-01-06)
Cell adhesion proteins of the Cadm (SynCAM/Necl) family regulate myelination and the organization of myelinated axons. In the peripheral nervous system (PNS), intercellular contact between Schwann cells and their underlying axons is believed to be mediated by binding of glial
Soundara Rajan Thangavelu et al.
Annals of neurosciences, 18(2), 48-55 (2011-04-01)
Amyotrophic Lateral Sclerosis, in which motor neurons degenerate, leading to paralysis, not only the affected motor neurons, but the surrounding non-neuronal cells also contribute significantly to the disease. However, the disease mechanism is not known. In this study we have
Bacterial neurotoxic metabolites in multiple sclerosis cerebrospinal fluid and plasma.
Ntranos, et al.
Brain, 145, 569-583 (2023)
Veronique Amor et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(15), 5089-5098 (2014-04-11)
Clustering of Na(+) channels at the nodes of Ranvier is coordinated by myelinating glia. In the peripheral nervous system, axoglial contact at the nodes is mediated by the binding of gliomedin and glial NrCAM to axonal neurofascin 186 (NF186). This
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