Anti-Nestin Antibody, clone 10C2, Cy3 conjugate

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

This antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.

Fusion protein corresponding to human Nestin.

Detect Nestin using this Anti-Nestin Antibody, clone 10C2, Cy3 conjugate validated for use in ICC.

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Evaluated by Immunocytochemistry in human RNcell CX neural stem cells (SCC007). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in human RNcell CX neural stem cells (SCC007).

The uncojugated parent antibody (Catalog No. MAB5326) has an observed MW at 200-220 kDa

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
RNcell CX neural stem cells (SCC007).

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