生物来源
mouse
质量水平
抗体形式
affinity purified immunoglobulin
抗体产品类型
primary antibodies
克隆
D8, monoclonal
种属反应性
eukaryotes
制造商/商品名称
Chemicon®
技术
western blot: suitable
同位素/亚型
IgG2a
运输
dry ice
靶向翻译后修饰
unmodified
特异性
Reacts with reduced or non-reduced glutathione on proteins only. Does not react with free glutathione.
免疫原
Glutathione-protein complexes.
应用
Anti-Glutathione Antibody, clone D8 is an antibody against Glutathione for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Western blotting of glutatione conjugated proteins under non-reduced conditions: 1:500-1:1000; does not react with glutatione by itself.
Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)
Optimal working dilutions must be determined by end user.
外形
Format: Purified
Immunoglobulin purified by protein A affinity and presented as a liquid in PBS, pH 7.2 with 0.01% sodium azide.
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Glutathione S-transferases interact with AMP-activated protein kinase: evidence for S-glutathionylation and activation in vitro.
Klaus, A; Zorman, S; Berthier, A; Polge, C; Ramirez, S; Michelland, S; Seve, M; Vertommen et al.
Testing null
Yugo Tsuchiya et al.
The Biochemical journal, 474(14), 2489-2508 (2017-03-28)
Coenzyme A (CoA) is an obligatory cofactor in all branches of life. CoA and its derivatives are involved in major metabolic pathways, allosteric interactions and the regulation of gene expression. Abnormal biosynthesis and homeostasis of CoA and its derivatives have
Omid Azimzadeh et al.
Cancers, 15(13) (2023-07-14)
Recent epidemiologic studies support an association between chronic low-dose radiation exposure and the development of cardiovascular disease (CVD). The molecular mechanisms underlying the adverse effect of chronic low dose exposure are not fully understood. To address this issue, we have
Vladimir A Mitkevich et al.
Oxidative medicine and cellular longevity, 2016, 9092328-9092328 (2016-05-31)
Many viruses induce oxidative stress and cause S-glutathionylation of Cys residues of the host and viral proteins. Changes in cell functioning during viral infection may be associated with glutathionylation of a number of key proteins including Na,K-ATPase which creates a
S-glutathionylation of the Na,K-ATPase catalytic ? subunit is a determinant of the enzyme redox sensitivity.
Petrushanko, IY; Yakushev, S; Mitkevich, VA; Kamanina, YV; Ziganshin, RH; Meng et al.
The Journal of Biological Chemistry null
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