生物来源
mouse
质量水平
偶联物
ALEXA FLUOR™ 555
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
NE14, monoclonal
种属反应性
mouse, rat, pig
种属反应性（根据同源性预测）
human (100% sequence homology), porcine (immunogen homology)
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
一般描述
神经丝是一种中间丝，可作为支持轴突细胞质的细胞骨架的主要元素。它们是轴突中最丰富的原纤维成分，平均频率是轴突微管的3-10倍。神经丝（直径10nm）由三个相互缠绕的原纤维构建而成，原纤维本身由单体蛋白质的两个四聚体原丝复合物组成。神经丝三联体蛋白（68/70、160和200 kDa）同时存在于中枢神经系统和周围神经系统中，通常是神经元特异性的。68/70 kDa NF-L蛋白可以自组装成丝状结构，但是160 kDa NF-M和200 kDa NF-H蛋白需要68/70 kDa NF-L蛋白的存在才能共组装。神经瘤、神经节瘤、神经节神经胶质瘤、神经节神经母细胞瘤和神经母细胞瘤的神经丝染色呈阳性。尽管通常仅限于神经元，但已在神经节旁瘤以及肾上腺和肾上腺嗜铬细胞瘤中检测到神经丝。类癌、皮肤神经内分泌癌和肺燕麦细胞癌也表达神经丝。有关更多神经丝信息和表位图谱，请在我们的网站上搜索“neurofilament（神经丝）”。
应用
使用经验证可用于ICC&IHC的抗神经丝200kDa抗体（克隆NE14，Alexa Fluor 555结合物）检测神经丝200kDa。
质量
通过免疫细胞化学在大鼠E18原代皮层细胞中进行评估。
免疫细胞化学分析：该抗体以1:100稀释度在大鼠E18原代皮层细胞中检测到神经丝。
免疫细胞化学分析：该抗体以1:100稀释度在大鼠E18原代皮层细胞中检测到神经丝。
其他说明
已证明未结合的抗神经丝可用于多种物种，包括人和猪。
法律信息
ALEXA FLUOR is a trademark of Life Technologies
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Inflammatory responses are crucial for regeneration following peripheral nerve injury (PNI). PNI triggers inflammatory responses at the site of injury. The DNA-sensing receptor cyclic GMP-AMP synthase (cGAS) and its downstream effector stimulator of interferon genes (STING) sense foreign and self-DNA
Yongchen Cui et al.
Anesthesiology, 139(6), 782-800 (2023-09-05)
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X Zhao et al.
Oncogene, 35(27), 3565-3576 (2015-11-10)
Previous studies have indicated the important roles of MYCN in tumorigenesis and progression of neuroblastoma (NB), the most common extracranial solid tumor derived from neural crest in childhood. However, the regulatory mechanisms of MYCN expression in NB still remain largely
Marie-Pier Girouard et al.
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In contrast to neurons in the CNS, damaged neurons from the peripheral nervous system (PNS) regenerate, but this process can be slow and imperfect. Successful regeneration is orchestrated by cytoskeletal reorganization at the tip of the proximal axon segment and
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Motor neuron degeneration and neuroinflammation are the most striking pathological features of amyotrophic lateral sclerosis (ALS). ALS currently has no cure and approved drugs have only a modest clinically therapeutic effect in patients. Drugs targeting different deleterious inflammatory pathways in
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