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主要文件

MAB4418

Sigma-Aldrich

抗-无刚毛鳞甲同系物2抗体,克隆7E2

clone 7E2, from mouse

别名:

achaete-scute complex (Drosophila) homolog-like 2, achaete-scute complex homolog 2 (Drosophila), achaete-scute complex homolog-like 2, achaete-scute complex-like 2, achaete-scute complex-like 2 (Drosophila), mammalian achaete/scute homologue 2

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

7E2, monoclonal

种属反应性

human

技术

ChIP: suitable
immunocytochemistry: suitable
western blot: suitable

同位素/亚型

IgG2bκ

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... ASCL2(430)

一般描述

Achaete Scute同系物2(也称为哺乳动物无刚毛鳞甲同系物2或Mash2)是在发育中的胎盘的绒毛外滋养细胞中特异性表达的核蛋白。胎盘发育涉及基本螺旋-环-螺旋转录因子Achaete Scute同系物2的控制,其可以调节海绵滋养细胞形成中的HIF(缺氧)。Achaete Scute同系物2的定向诱变产生功能丧失导致妊娠中期胚胎致死,这是由于胎盘衰竭与海绵滋养细胞缺乏和迷宫滋养细胞层减少有关。除了健康的胎盘发育外,Achaete Scute蛋白还可能参与中枢和周围神经系统神经元前体的测定。Achaete Scute同系物2也被认为是肠干细胞命运的控制者,并且是维持成人肠干细胞所必需的(Tanaka,M.,et al.,(1997)Dev Biol. 190(10):55-65)。

特异性

该抗体识别C末端附近的Achaete Scute同系物2。

免疫原

His标记的重组蛋白,对应于C末端附近的人Achaete Scute同系物2。

应用

免疫细胞化学分析: 一个先前批次被独立实验室用于IC(Van de Flier,L.G.,et al.,2009)

染色质免疫沉淀分析: 一个先前批次被独立实验室用于ChIP(Hatzis,P.,et al.,2008)。
抗Achaete Scute同系物2抗体,克隆7E2检测Achaete Scute同系物2的水平,&已发布&验证可用于WB、IC、ChIP。

质量

通过蛋白质印迹法在人胎盘组织裂解物中进行评价。

蛋白质印迹分析:1 µg/mL的该抗体在10 µg人胎盘组织裂解物上检测到Achaete Scute同系物2。

目标描述

约20 kDa

外形

形式:纯化

其他说明

浓度:关于批次特定浓度请参见检验报告。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Stem cells generate rapidly dividing transit-amplifying cells that have lost the capacity for self-renewal but cycle for a number of times until they exit the cell cycle and undergo terminal differentiation. We know very little of the type of signals
Yangyang Shang et al.
Oncotarget, 6(31), 30993-31006 (2015-08-27)
The role of Achaete scute-like 2 (Ascl2) in colorectal cancer (CRC) cell differentiation is unknown. LS174T, HT-29 and Caco-2 cells have high Ascl2 expression, while Lovo and SW480 cells have low Ascl2 expression. LS174T and HT-29 cells with Ascl2 knockdown
Jun Ye et al.
Cell cycle (Georgetown, Tex.), 17(8), 1014-1025 (2018-06-12)
The Wnt signaling pathway controls stem cell identity in the intestinal epithelium and cancer stem cells (CSCs). The transcription factor Ascl2 (Wnt target gene) is fate decider of intestinal cryptic stem cells and colon cancer stem cells. It is unclear
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Trophoblast stem cell (TSC) is crucial to the formation of placenta in mammals. Histone demethylase JMJD2 (also known as KDM4) family proteins have been previously shown to support self-renewal and differentiation of stem cells. However, their roles in the context

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