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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
17A6.1, monoclonal
种属反应性
human
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG2aκ
NCBI登记号
UniProt登记号
1 of 4
此商品 | SRP6166 | H1404 | SRP6253 |
|---|---|---|---|
| biological source human | biological source human | biological source human | biological source human |
| recombinant expressed in CHO cells | recombinant expressed in HEK 293 cells | recombinant expressed in Baculovirus infected High-5 cells | recombinant expressed in HEK 293 cells |
| technique(s) cell culture | embryo: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| assay ≥97% (HPLC), ≥97% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay >95% (SDS-PAGE) | assay ≥97% (SDS-PAGE) |
| mol wt 80.017 kDa | mol wt 70 kDa (single chain, glycosylated) | mol wt ~80 kDa | mol wt calculated mol wt 6.3 kDa, observed mol wt 6.3 kDa by SDS-PAGE (DTT-reduced.) |
| form lyophilized | form lyophilized | form lyophilized powder | form lyophilized powder |
一般描述
Prominin-1（UniProt：O43490；也称为抗原AC133，Prominin样蛋白质1，CD133）由人的PROM1（也称为PROML1，MSTP061）基因（基因ID：8842）编码。CD133（Prominin-1）是多通道细胞表面糖蛋白，在细胞分化、增殖和凋亡中起作用。它结合含胆固醇的质膜微区中的胆固醇，并可能在上皮细胞的顶质膜的组织中起作用。在视网膜早期发育过程中，CD133充当盘形态发生的关键调节因子。已知PROM1基因缺陷会导致色素性视网膜炎、视锥杆细胞营养不良和视网膜黄斑营养不良2。已经报道了几种癌细胞类型中较高水平的CD133，包括各种白血病、视网膜母细胞瘤、胶质母细胞瘤和肾癌。在胰腺癌、胃癌、结直肠癌和肝细胞癌中也观察到更高水平的CD133。它也被认为是成年小肠中干细胞的标志物，这些干细胞易于转化为保留一部分突变Prom1+肿瘤细胞的肿瘤。（参考文献：Zhu, L., et al. (2009).Nature 477（7229）；603-607）。
特异性
克隆17A6.1通过蛋白质印迹法检测重组CD133（Prominin-1），还通过免疫细胞化学检测U251细胞中的CD133（Prominin-1）。
免疫原
GST标记的重组片段，对应于人CD133（Prominin-1）N末端区域的77个氨基酸。
应用
使用该小鼠单克隆抗CD133（Prominin-1）抗体（克隆17A6.1，目录号:编号MAB4399-I）检测CD133。经验证可用于免疫细胞化学。
免疫细胞化学分析：一个代表性批次以1:50稀释度在U251细胞中检测到CD133。
质量
通过重组Prominin-1的蛋白质印迹法进行评估。
蛋白质印迹分析：1 µg/mL的该抗体检测到2.5 µg重组Prominin-1蛋白。
蛋白质印迹分析：1 µg/mL的该抗体检测到2.5 µg重组Prominin-1蛋白。
目标描述
观测分子量〜97 kDa；计算分子量97.2 kDa。在某些裂解物中可以观察到未鉴定的条带。
外形
形式：纯化
其他说明
浓度：请参考特定批次的数据表。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
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