Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Reactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys (Baboon, Rhesus monkey, Mangabey, Mandrill, Celebese Black Ape, African Green Monkey), Bovine, Canine, Goat, Guinea Pig, Hamster, Mouse, Rat and Sheep. This antibody does not cross react with other isozymes of AP.

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.

2102Ep human Embryonal Carcinoma Cells

Epitope: Liver/Bone/Kidney Alkaline Phosphatase

Immunocytochemistry: A previous lot was used on IC.

Immunofluorescence: A previous lot of this antibody was used in IF.

FACS Analysis: A starting range of 5-20 µg/mL is suggested.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E is validated for use in FC, IF, IC, IP for the detection of TRA-2-49.

212/80/55 kDa

Protein A purified

Purified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide

Stable for 1 year at from date of receipt.

Control
Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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