跳转至内容
Merck
CN

MAB4310

抗-CD133抗体，克隆13A4

clone 13A4, Chemicon®, from rat

别名:

Prominin-1, AC133 (human)

登录 查看组织和合同定价。

选择尺寸

关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

抗-CD133抗体，克隆13A4, clone 13A4, Chemicon®, from rat

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

13A4, monoclonal

species reactivity

mouse

should not react with

chicken, Drosophila, rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

input

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)

isotype

IgG1κ

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_006017.1

UniProt accession no.

O43490

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

dog ... Prom1(488816)
human ... PROM1(8842)
mouse ... Prom1(19126)
rat ... Prom1(60357)

相关类别

Analysis Note

对照
阳性对照：肾组织或肾膜
阴性对照：肝组织或肝膜

Application

抗CD133抗体，克隆13A4可检测CD133的水平，&已发布&验证可用于FC、IH、IP & WB。

蛋白质印迹分析：代表性批次的1:1000稀释液在猴肾组织切片中检测到CD133。
研究子类别
神经干细胞

造血干细胞
研究类别
干细胞研究

Biochem/physiol Actions

识别小鼠Prominin-1（有时也称为CD133，在人同源基因序列的情况下，称为AC133），一种115-120 kDa五跨膜（5-TM）结构域糖蛋白。Prominin-1在原始细胞上表达，例如造血干细胞和祖细胞、神经&内皮干细胞、视网膜和视网膜母细胞瘤，以及发育中的上皮细胞。迄今为止，Prominin-1的功能和配体尚不清楚。

该抗体可用于从大脑和骨髓中分离鼠干细胞。

13A4抗体不与大鼠、人、鸡或果蝇抗原发生交叉反应。 论文原文位于：http://www.pnas.org/cgi/reprint/94/23/12425.pdf

Disclaimer

除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

General description

115 kDa
Prominin是一种新型质膜蛋白，具有N末端胞外域，位于两个短胞质环和两个大糖基化胞外域两侧的五个跨膜区段以及一个胞质C末端结构域。秀丽隐杆线虫的DNA序列预测存在具有相同特征的蛋白质，这表明prominin在脊椎动物和无脊椎动物之间是保守的。Prominin不仅存在于小鼠胚胎的神经上皮细胞中，而且还存在于小鼠胚胎的各种其他上皮细胞中。在成年小鼠中，已在脑室管膜层和肾小管中检测到prominin。在这些上皮细胞中，prominin特异于顶端表面，在那里它选择性地与微绒毛和微绒毛相关结构相关。值得注意的是，在CHO细胞中表达后，prominin优先定位于质膜突起，例如丝状伪足、片状伪足和微端丝。这些观察结果暗示，突出蛋白包含针对质膜突起而不是平面细胞表面和/或保留在质膜突起中的信息。此外，结果表明，将膜蛋白靶向上皮细胞的微绒毛和非上皮细胞的质膜突起的机制高度相关。{Weigmann, A et al.1997}.另请参阅{http://www.ncbi.nlm.nih.gov/prow/guide/438375806_g.htm}以获取有关CD133和prominin表达的更多信息。

Immunogen

将主要由神经上皮组成的十个12日龄的NMRI小鼠胚胎（E12，〜1 mg蛋白）的端脑在1 ml PBS中匀浆，与压碎的硝酸纤维素滤膜（在0.5 ml PBS中为1 cm2）混合作为佐剂，以及腹腔注射至一只LouXSD大鼠。{Weigmann et al, PNAS 1997}

Other Notes

浓度：关于批次特定浓度请参见检验报告。

Physical form

形式：纯化
纯化蛋白A
蛋白A纯化的小鼠免疫球蛋白，溶于20 mM磷酸钠、250 mM NaCl（pH.7.6）中，含0.1%叠氮化钠作为防腐剂。

Preparation Note

自发运之日起，在2–8°C条件下可稳定保存1年。为了最大程度地回收产品，需在融化后和取下盖子之前将原始样品管进行离心。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

未找到合适的产品？  

试试我们的产品选型工具.

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Zhibo Xia et al.
Molecular cancer research : MCR, 11(9), 1101-1111 (2013-06-14)
Glioma-initiating cells (GIC), which are characterized by their self-renewal capacity and tumorigenicity, were recently identified as a highly tumorigenic subpopulation of glioblastoma multiforme and are considered responsible for glioblastoma recurrence and chemo/radiation resistance. Previously, it was revealed that Wnt signaling
Activation of the sonic hedgehog signaling pathway occurs in the CD133 positive cells of mouse liver cancer Hepa 1-6 cells.
Jeng, KS; Sheen, IS; Jeng, WJ; Yu, MC; Hsiau, HI; Chang, FY; Tsai, HH
OncoTargets and Therapy null
Ectopic expression of the WWOX gene suppresses stemness of human ovarian cancer stem cells.
Yan, HC; Xu, J; Fang, LS; Qiu, YY; Lin, XM; Huang, HX; Han, QY
Oncology Letters null
Taofeek K Owonikoko et al.
Journal of translational medicine, 14(1), 111-111 (2016-05-05)
SCLC has limited treatment options and inadequate preclinical models. Promising activity of arsenic trioxide (ASO) recorded in conventional preclinical models of SCLC supported the clinical evaluation of ASO in patients. We assessed the efficacy of ASO in relapsed SCLC patients
Regional differences in stem cell/progenitor cell populations from the mouse achilles tendon.
Mienaltowski, MJ; Adams, SM; Birk, DE
Tissue Engineering: Part A null
查看所有相关文献

相关内容

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

ANTI- PROMININ-1(CD133)
PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持