MAB427-C
Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free
clone 1A, from mouse
别名:
Voltage-dependent L-type calcium channel subunit alpha-1S, Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Voltage-gated calcium channel subunit alpha Cav1.1
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About This Item
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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
1A, monoclonal
种属反应性
plant, human, rabbit, mouse
技术
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... PPP1R15A(23645)
一般描述
Voltage-dependent L-type calcium channel subunit alpha-1S (UniProt P07293; also known as Calcium channel, L type, alpha-1 polypeptide, isoform 3, skeletal muscle, Voltage-gated calcium channel subunit alpha Cav1.1) is encoded by the CACNA1S (also known as CACH1, CACN1, CACNL1A3) gene (Gene ID 100009585) in Oryctolagus cuniculus (rabbit) species. Voltage-dependent calcium channels (VDCC) are found in the membrane of excitable cells (e.g., muscle, glial cells, neurons) and exhibit 1000-fold greater permeability to calcium than to sodium under normal physiological conditions. There exist five types of VDCCs, including four high voltage-activated channels (Neural- or N-type channel, Residual- or R-type channel, Purkinje- or P/Q-type channel, and the dihydropyridine-sensitive Long-lasting or L-type channels) and the low voltage-activated Transient or T-type calcium channels. VDCCs exist as a complex of different subunits (α1, α2δ, β1-4, and γ), with α1 being the the ion-conducting pore-forming subunit that contains voltage-sensing machinery and the drug/toxin-binding sites. There exist ten genes coding for different α1 subunits, four of which code for L-type alpha-1 subunits (alpha-1A/Cav2.1/CACNA1A, alpha-1C/Cav1.2/CACNA1C, alpha-1D/Cav1.3/CACNA1D, and alpha-1S/Cav1.1/CACNA1S). The dihydropyridine-sensitive L-type channels are responsible for excitation-contraction coupling of skeletal, smooth, and cardiac muscle and for hormone secretion in endocrine cells. The α1S subunit (Cav1.1) contains the characteristic four homologous I–IV domains (a.a. 38-337, 418-664, 786-1068, 1105-1384) with six transmembrane α-helices each, having both its N- and C-terminal ends exposed intracellularly (a.a. 1-51, 1382-1873).
特异性
Clone 1A targets the extracellular region of voltage-dependent L-type calcium channel subunit alpha-1S or Cav1.1 encoded by the CACNA1S gene.
免疫原
Dihydropyridine-binding complex isolated from rabbit muscle t-tubules.
Epitope: Extracellular domain.
应用
Anti-Cav1.1 alpha1s Antibody, clone 1A, Ascites Free is an antibody against Cav1.1 alpha1s for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence, Immunoprecipitation.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
质量
Evaluated by Western Blotting in Jurkat membrane extract.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cav1.1 alpha1s in 10 µg of Jurkat membrane extract.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cav1.1 alpha1s in 10 µg of Jurkat membrane extract.
目标描述
~212 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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