Anti-CD34 Class I Antibody, clone B1-3C5

This antibody belongs to CD34 (assigned by the Third International Workshop on Leukocyte Differentiation Antigens, Oxford, 1987; Hogg & Horton, 1987). It reacts with a 120kD glycoprotein found on progenitor cell populations in human bone marrow and foetal liver (Katz et al., 1985). This antigen is normally restricted to immature human haemopoietic precursor cells (Peschel & Koller, 1989).

Cell reactivity (Tindle et al., 1985):

Normal: No reaction with normal peripheral lymphocytes, granulocytes, monocytes, erythrocytes and platelets. Reacts with <4% of cells in normal bone marrow.

Clinical: Reaction

Acute myeloid leukemia (AML, M1 and M2) positive

Null lymphoblastic leukemia (Null ALL) positive

Common acute lymphoblastic leukemia (CALL) positive

Myeloblastic and lymphoblastic crises of chronic

granulocytic leukemia (CGL) positive

Chronic myeloid leukemia (CML) negative

T cell acute lymphoblastic leukemia (TALL) negative

B cell acute lymphoblastic leukemia (BALL) negative

Chronic lymphocytic leukemia (CLL) negative

Cell lines:

Reacts with early myeloblast line KG1. No reaction with null, B or T lymphoid, erythroid or later myeloid cell lines.

KG1 human acute myelogenous cell line.

Detect CD34 Class I using this Anti-CD34 Antibody (Class 1), clone B1-3C5 validated for use in FC & IF.

Identification of CD34 positive cells is important for the diagnosis and classification of acute myeloid leukemia (AML). It is especially useful in patients with poorly differentiated myeloblasts, as well as in myeloblastic crises of chronic granulocytic leukemia. It has been recommended that this antibody be used as part of a panel for screening of leukemia (Batinic et al., 1989).

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:25

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) or bone marrow cells in 100 μl of buffer.

2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Format: Purified

The antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. Product stable for 6 months when stored as directed.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

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