产品名称
抗-53BP1抗体，克隆BP13, clone BP13, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BP13, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TP53BP1(7158)
Application
用IF&WB中验证的抗53BP1抗体（克隆BP13（小鼠单克隆抗体））检测53BP1（也称为p53结合蛋白1）。
研究子类别
细胞周期、DNA复制&修复
细胞周期、DNA复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
蛋白质印迹法：识别>250 kD的蛋白质。建议的稀释缓冲液为TBST。建议的封闭缓冲液为5%脱脂奶粉。
免疫沉淀。免疫印迹的预期大小为>250 kD。建议的裂解缓冲液为NETN。建议的捕获剂是蛋白A+兔抗小鼠接头抗体。
最佳工作稀释度必须由最终用户进行确定。
免疫沉淀。免疫印迹的预期大小为>250 kD。建议的裂解缓冲液为NETN。建议的捕获剂是蛋白A+兔抗小鼠接头抗体。
最佳工作稀释度必须由最终用户进行确定。
Biochem/physiol Actions
53BP1
Disclaimer
除非我们的目录或产品随附的其他公司文件中另有说明，否则我们的产品预期仅用于研究用途，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。
General description
P53结合蛋白1（53BP1）在肿瘤抑制中起关键作用，并且是ATM激酶的假定底物。DNA损伤后，它被磷酸化并重新定位到损伤的推测位点，特别是双链断裂。这也暗示了在DNA修复和维持基因组稳定性中的作用。它是序列特异性转录因子，在哺乳动物细胞对遗传毒性应激的反应中起着核心作用。同样，53BP1定位于有丝分裂细胞中的动植物，表明在有丝分裂检查点信号传导中具有潜在功能。
Immunogen
重组人53BP1蛋白。
Physical form
形式：纯化
纯化的免疫球蛋白。PBS中的液体，含有叠氮化钠。
Preparation Note
自接收之日起，以未稀释的等分试样在2-8°C下保存长达12个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Ankana Tiwari et al.
Nature communications, 9(1), 677-677 (2018-02-16)
Chromosome missegregation acts as one of the driving forces for chromosome instability and cancer development. Here, we find that in human cancer cells, HeLa and U2OS, depletion of 53BP1 (p53-binding protein 1) exacerbates chromosome non-disjunction resulting from a new type
Shankar J Chinta et al.
Cell reports, 22(4), 930-940 (2018-02-02)
Exposure to the herbicide paraquat (PQ) is associated with an increased risk of idiopathic Parkinson's disease (PD). Therapies based on PQ's presumed mechanisms of action have not, however, yielded effective disease therapies. Cellular senescence is an anticancer mechanism that arrests
Shuang Liu et al.
FEBS open bio, 9(2), 304-314 (2019-02-15)
RNA splicing has emerged as a critical player in the DNA damage response (DDR). However, the underlying mechanism(s) by which pre-mRNA splicing is coordinately regulated by genotoxic stress has remained largely unclear. Here, we show that a DDR factor, DNA-dependent
Grasiella Angelina Andriani et al.
Scientific reports, 6, 35218-35218 (2016-10-13)
Age-related accumulation of ploidy changes is associated with decreased expression of genes controlling chromosome segregation and cohesin functions. To determine the consequences of whole chromosome instability (W-CIN) we down-regulated the spindle assembly checkpoint component BUB1 and the mitotic cohesin SMC1A
Heather A Galick et al.
Oncotarget, 8(49), 85883-85895 (2017-11-22)
Base excision repair (BER) is a key genome maintenance pathway. The NEIL1 DNA glycosylase recognizes oxidized bases, and likely removes damage in advance of the replication fork. The rs5745906 SNP of the NEIL1 gene is a rare human germline variant
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持