推荐产品
生物来源
mouse
质量水平
偶联物
ALEXA FLUOR™ 555
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
A60, monoclonal
种属反应性
rat, mouse
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
同位素/亚型
IgG1
运输
wet ice
1 of 4
此商品 | MAB377C3 | ABN78A4 | ABN78C3 |
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| Gene Information mouse ... Rbfox3(52897) | Gene Information mouse ... Rbfox3(52897) | Gene Information mouse ... Rbfox3(52897) | Gene Information mouse ... Rbfox3(52897) |
| conjugate ALEXA FLUOR™ 555 | conjugate CY3 conjugate | conjugate ALEXA FLUOR™ 488 | conjugate CY3 conjugate |
| species reactivity rat, mouse | species reactivity rat | species reactivity rat, mouse | species reactivity rat, mouse |
| clone A60, monoclonal | clone A60, monoclonal | clone polyclonal | clone polyclonal |
| biological source mouse | biological source mouse | biological source rabbit | biological source rabbit |
一般描述
NeuN抗体（神经元核；克隆A60）特异性识别DNA结合的神经元特异性蛋白NeuN，该蛋白存在于所有检验的脊椎动物的大多数CNS和PNS神经元细胞类型中。NeuN蛋白的分布明显局限于胎儿和成人大脑中的神经元细胞核、核周体和一些近端神经元突起，但有些神经元在所有年龄段都不能被NeuN识别：例如，INL视网膜细胞、Cajal Retzius细胞、Purkinje细胞、下橄榄核和齿状核神经元以及交感神经节细胞（Mullen等人，1992；Wolf等人，1996）。免疫组织化学可检测到的NeuN蛋白首先出现在与神经元退出细胞周期和/或神经元终末分化开始相对应的发育时间点（Mullen等人，1992）。免疫反应性出现在小鼠神经管中的E9.5附近，并在E12.5的整个发育中的神经系统中广泛存在。强烈的核染色表明核调节蛋白功能。但是，目前尚无关于NeuN蛋白抗原是否在远端细胞质中具有功能或在转运回细胞核之前仅在那里合成的证据。从纯化的细胞核和全脑提取物中分离的蛋白质在免疫印迹上没有发现差异（Mullen等人，1992）。
应用
免疫组织化学分析： 一个代表性批次以1:100稀释度在成年小鼠脑冷冻切片中检测到NeuN。
该抗NeuN抗体（克隆A60，Alexa Fluor 555结合物）经验证可用于ICC、IHC检测NeuN。
质量
通过免疫细胞化学在大鼠E18原代皮层细胞中进行评估。
免疫细胞化学分析：该抗体以1:100稀释度在大鼠E18原代皮层细胞中检测到NeuN。
免疫细胞化学分析：该抗体以1:100稀释度在大鼠E18原代皮层细胞中检测到NeuN。
目标描述
未偶联的亲本抗体（目录号MAB377）的分子量在46-48 kDa范围内，可能在约66 kDa处有另一条条带
其他说明
已证明未结合的抗NeuN抗体可用于多种物种，包括人、雪貂、鸡和禽类。
法律信息
ALEXA FLUOR is a trademark of Life Technologies
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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