推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
SMP14, monoclonal
种属反应性
rat, human, mouse
制造商/商品名称
Chemicon®
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
同位素/亚型
IgG1
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(314856)
一般描述
MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
特异性
Recognizes human MDM2.
免疫原
Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.
应用
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
外形
Format: Purified
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide
分析说明
Control
POSITIVE CONTROL: Breast carcinoma
POSITIVE CONTROL: Breast carcinoma
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Richard O Adeyemi et al.
Journal of virology, 86(15), 8328-8332 (2012-05-25)
The DNA damage response to infection with minute virus of mice (MVM) leads to activated p53; however, p21 levels are reduced via a proteasome-mediated mechanism. This loss was sustained, as virus replicated in infected cells held at the G(2)/M border.
Gengze Wu et al.
Circulation, 130(17), 1452-1465 (2014-08-27)
Long noncoding RNAs (lncRNAs) have recently been implicated in many biological processes and diseases. Atherosclerosis is a major risk factor for cardiovascular disease. However, the functional role of lncRNAs in atherosclerosis is largely unknown. We identified lincRNA-p21 as a key
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