Anti-Chloramphenicol Acetyltransferase Antibody, clone CAT-1

Chloramphenicol acetyltransferase (UniProt: P62577; also known as EC:2.3.1.28, CAT) is encoded by the Cat gene (Gene ID: 59683728) in E. coli. Chloramphenicol acetyltransferase is an enzyme that catalyzes the acetyl-S-CoA-dependent acetylation of chloramphenicol at the 3-hydroxyl group leading to the production of 3-acetoxy chloramphenicol that does not bind to bacterial ribosomes and does not inhibit peptidyltransferase activity. It displays high stability in cells and has a half-life of about 48 hours. A highly reactive histidine residue has been implicated in the catalytic mechanism. CAT is shown to be an effector of chloramphenicol resistance in bacteria. Its synthesis is shown to be constitutive in E. coli and other Gram-negative bacteria that harbor plasmids bearing the structural gene for the enzyme. However, in Gram-positive bacteria such as staphylococci and streptococci it is synthesized only in the presence of chloramphenicol and related compounds. (Ref.:Shaw WH. (1983). CRC Crit. Rev. Biochem. 14(1); 1-46).

This mouse monoclonal Anti-Chloramphenicol Acetyltransferase, clone CAT-1, Cat. No. MAB3678-I is tested for use in Immunocytochemistry and Western Blotting and Immunofluorescence, for the detection of Chloramphenicol acetyltransferase.

Clone CAT-1 is a mouse monoclonal antibody that detects Chloramphenicol acetyltransferase.

Full-length recombinant chloramphenicol acetyl transferase.

Quality Control Testing

Evaluated by Immunocytochemistry in HEK293 cells transfected with gWIZ CAT plasmid.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Chloramphenicol Acetyltransferase in HEK293 cells transfected with gWIZ CAT plasmid.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Chloramphenicol acetyltransferase in Immunofluorescence applications (Markus, B.M., et al. (2019). mSphere.;4(3):e00386-19).

Western Blotting Analysis: A representative lot detected Chloramphenicol acetyltransferase in Western Blotting applications (Smith, M.C., et al. (2011). Antiviral Res.;91(3):259-66; Markus, B.M., et al. (2019). mSphere.;4(3):e00386-19).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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