Anti-Fascin Antibody, clone 55K2

Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.

Human fascin

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.

Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.

Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.

Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.

Optimal working dilutions must be determined by the end user.

Format: Purified

Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.

Control
POSITIVE CONTROL:

Hela cells, neuronal tissues, antigen presenting dendritic cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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