biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
483.15, monoclonal
species reactivity
rat, human, monkey, bovine, mouse
species reactivity (predicted by homology)
all
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
isotype
IgMκ
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... OGG1(4968)
General description
Immunogen
Application
免疫组织化学分析:一个代表性批次通过荧光免疫组织化学检测到诱导表达突变线粒体同种型尿嘧啶– DNA 糖基化酶的小鼠石蜡包埋冠状海马切片中核 8-氧代鸟嘌呤 (8-oxoG)的免疫反应性增加(Lauritzen, K.H., et al. (2011).DNA Repair (Amst).10(6):639-653)。
免疫组织化学分析:一个代表性批次通过荧光免疫组织化学检测到,在接触卵毒性剂 4-乙烯基环己烯二环氧化物(VCD)、甲氧氯(MXC)或甲萘醌(MEN) 后,新生小鼠切除的卵巢中核 8-氧代鸟嘌呤 (8-oxoG) 免疫反应性显著增加(Sobinoff, A.P., et al. (2010).Toxicol.Sci. 118(2):653-666)。
免疫细胞化学分析:一个代表性批次检测到,在 IL-1beta 或 ROS 诱导剂甲萘醌处理后RINm5F 大鼠胰岛瘤细胞中 8-氧代鸟嘌呤 (8-oxoG) 免疫反应性显著增加(Mehmeti, I., et al. (2011).Biochim.Biophys.Acta.1813(10):1827-1835)。
免疫细胞化学分析:一个代表性批次检测到,在被被血鞭毛虫原生动物寄生虫克氏锥虫感染后,AC16 人心肌细胞中的核 8-氧代鸟嘌呤 (8-oxoG)免疫反应性显著增加 (Ba, X., et al. (2010).J. Biol. Chem. 285(15):11596-11606)。
免疫细胞化学分析:代表性批次通过通过荧光免疫组织化学检测到营养剥夺诱导的 HeLa 和 COS-7 细胞核 8-氧代鸟嘌呤 (8-oxoG) 免疫反应性增强(Conlon, K.A., et al. (2003).DNA Repair (Amst).2(12):1337-1352; Conlon, K.A., et al. (2000).J. Histotechnol.23(1):37-44)。
ELISA分析:通过竞争性ELISA证实了一个代表性批次对8-氧代鸟嘌呤的特异性。仅当浓度增加到毫摩尔范围时才观察到dGMP、dAMP、dCMP 或 TMP 的竞争。
Biochem/physiol Actions
Physical form
Analysis Note
免疫细胞化学分析:该抗体的1:50稀释液在HeLa细胞中检测到8-氧代鸟嘌呤。
Other Notes
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
"Mods – modifications – small alterations can have a big impact on form and function. In the motorsports world, stock vehicles are modified to enhance their performance. Modifications, or mods, to the engine, drive train, intake and exhaust components add up to provide phenomenal performance gains that can be measured as horsepower and torque increases, which yield a competitive advantage, and result in reduced run times. In biology, proteins undergo modifications that alter their function. Some proteins require the modifications in order to perform their function effectively, whether it’s a pro-protein that is cleaved to produce an active enzyme, or a protein that is phosphorylated to facilitate a signaling process. Other proteins, such as histones, undergo modifications that regulate gene expression and alter cellular function. There are several post translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination that impact protein function and activity. As a result, the analysis of proteins and their post-translational modifications are particularly important for the study of normal and disease-associated processes. New antibodies to detect phospho Histidines are now available from EMD Millipore."
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB3560-C | 04055977296419 |