MAB3516
Anti-Heat Shock Protein 70 Antibody, clone W27
clone W27, Chemicon®, from mouse
别名:
HSP70
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
W27, monoclonal
Application:
IHC, IP, WB
Citations:
7
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
W27, monoclonal
species reactivity
bovine, human, monkey
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSPA4(3308)
Immunogen
HSP70 from HeLa cells.
Application
Immunoblotting: 0.5-1 μg/mL
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)
Flow cytometry
Optimal working dilutions must be determined by end user.
This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.
Biochem/physiol Actions
Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
POSITIVE CONTROL:
HeLa cells, BT474 cells or breast carcinoma.
Physical form
Format: Purified
Analysis Note
Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
相关内容
MOUSE ANTI-HEAT SHOCK PROTEIN 70 (HSP70) MONOCLONAL ANTIBODY
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Zulli, A; Lau, E; Wijaya, BP; Jin, X; Sutarga, K; Schwartz, GD; Learmont, J; Wookey et al.
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Neurobiology of aging, 58, 239-239 (2017-07-19)
Mutations in UBQLN2 have been associated with rare cases of X-linked juvenile and adult forms of amyotrophic lateral sclerosis (ALS) and ALS linked to frontotemporal dementia (FTD). Here, we report 1 known (c.1489C>T, p.Pro497Ser, P497S) and 3 novel (c.1481C>T, p.Pro494Leu
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB3516 | 04053252466021 |