推荐产品
生物来源
mouse
抗体形式
ascites fluid
克隆
SP14, monoclonal
种属反应性
pig, human, hamster, rat
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... SNAP25(6616)
特异性
SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.
免疫原
Crude synaptic immunoprecipitate (Human)
应用
Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
外形
Liquid. Contains no preservative.
储存及稳定性
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Matthew J Van Hook et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4618-4634 (2017-04-02)
G-protein βγ subunits (Gβγ) interact with presynaptic proteins and regulate neurotransmitter release downstream of Ca2+ influx. To accomplish their roles in sensory signaling, photoreceptor synapses use specialized presynaptic proteins that support neurotransmission at active zone structures known as ribbons. While
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Toshihiko Shirafuji et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(2), 278-290 (2017-11-24)
Protein kinase Cγ (PKCγ) knock-out (KO) animals exhibit symptoms of Parkinson's disease (PD), including dopaminergic neuronal loss in the substantia nigra. However, the PKCγ substrates responsible for the survival of dopaminergic neurons in vivo have not yet been elucidated. Previously
The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.
Hong, HK; Chakravarti, A; Takahashi, JS
Proceedings of the National Academy of Sciences of the USA null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
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