biological source
mouse
antibody form
ascites fluid
clone
SP14, monoclonal
species reactivity
pig, human, hamster, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SNAP25(6616)
Immunogen
Crude synaptic immunoprecipitate (Human)
Application
Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Biochem/physiol Actions
SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.
Physical form
Liquid. Contains no preservative.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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