生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
4F5, monoclonal
种属反应性
rat, rabbit
制造商/商品名称
Chemicon®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
rabbit ... Slc9A3(100009430)
rat ... Slc9A3(24784)
特异性
Na+/H+ exchanger, isoform NHE3.
免疫原
Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.
应用
Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.
Immunocytochemistry
Immunoprecipitation (use non-ionic detergent)
Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
联系
Replaces: MAB3134
外形
Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Yanling Yan et al.
Molecular and cellular biochemistry, 367(1-2), 175-183 (2012-05-24)
We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase α1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the α1 subunit
Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells.
Li, XC; Hopfer, U; Zhuo, JL
American Journal of Physiology: Renal Physiology null
Xiaochun Li et al.
Cells, 12(11) (2023-06-10)
The current prevailing paradigm in the renin-angiotensin system dictates that most, if not all, biological, physiological, and pathological responses to its most potent peptide, angiotensin II (Ang II), are mediated by extracellular Ang II activating its cell surface receptors. Whether
Elevated BSC-1 and ROMK expression in Dahl salt-sensitive rat kidneys.
Hoagland, KM; Flasch, AK; Dahly-Vernon, AJ; dos Santos, EA; Knepper, MA; Roman, RJ
Hypertension null
Angiotensin II-mediated biphasic regulation of proximal tubular Na+/H+ exchanger 3 is impaired during oxidative stress.
Banday, AA; Lokhandwala, MF
American Journal of Physiology: Renal Physiology null
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