Anti-Surfactant Protein D Antibody, lectin domain, clone IVF11

Surfactant protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620 kDa under non-reducing conditions and 43 kDa under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A.

MAB3132 recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells (in which SpD is highly concentrated), SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells are negative. No reaction on rat skin, colon, kidney or liver. SP-A cross reacitivity is typically evident at very low dilutions (1 to less than a few hundred) by ELISA.

Epitope: lectin domain

Purified rat surfactant protein D from bronchoalveolar lavage.

Immunohistochemistry on snap frozen or paraffin embedded tissue.

Suggested working dilution for fresh rat tissue is 1:400 and for fresh human tissue is 1:20. Fixation: acetone 5 min at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W for paraffin tissues.

Western blot

ELISA: as capture antibody

Optimal working dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Ion & Transport Channels

This Anti-Surfactant Protein D Antibody, lectin domain, clone IVF11 is validated for use in ELISA, IH(P), WB for the detection of Surfactant Protein D.

Replaces: MAB3130

Format: Purified

Immunoglobulin purified from tissue culture supernatant. Lyophilized from buffer containing 5 mg/mL BSA and 0.09% sodium azide.

Maintain lyophilized material at 2-8°C for up to 12 months from date of receipt. Reconstitute lyophilized material with 500 μL of sterile distilled water. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

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