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生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
M12.13, monoclonal
种属反应性
human, mouse, rat
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... GJB1(2705)
特异性
Monoclonal antibody MAB3069 was produced by the immunization of BALB/c mice with isolated rat junctional complexes, prepared by the method of Stevenson and Goodenough (1988). Epitope mapping, hydropathy plotting, and topological studies suggest that this antibody is very likely directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.
应用
Anti-Connexin 32 Antibody, clone M12.13 detects level of Connexin 32 & has been published & validated for use in WB, IH.
Immunohistochemistry: acetone or unfixed tissues recommended. Epitope is internal, paraffin sections are untested.
Works with lightly formaldehyde-fixed tissue.
Western blot: Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates.
Optimal working dilutions must be determined by end user.
Works with lightly formaldehyde-fixed tissue.
Western blot: Detects a 27 kDa Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47 kDa band corresponding to aggregates.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
外形
Format: Purified
Liquid in 0.02M PB, 0.25M NaCl, containing 0.1% sodium azide.
储存及稳定性
Maintain at 2-8°C in convenient undiluted aliquots for up to 6 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
W Matsuyama et al.
Journal of human genetics, 46(6), 307-313 (2001-06-08)
To clarify the pathomechanism in three patients with X-linked Charcot-Marie-Tooth disease (CMTX) and unique clinical features, we studied three connexin (Cx) 32 (GJB1) mutants with respect to cellular localization in cultured cells. Wild-type Cx32 and three Cx32 mutants (Va163Ile and
J E Rash et al.
Neuroscience, 147(4), 938-956 (2007-07-03)
Locus coeruleus neurons are strongly coupled during early postnatal development, and it has been proposed that these neurons are linked by extraordinarily abundant gap junctions consisting of connexin32 (Cx32) and connexin26 (Cx26), and that those same connexins abundantly link neurons
Lorena Rela et al.
Glia, 58(6), 665-678 (2009-12-10)
Olfactory ensheathing cells (OECs) have been repeatedly implicated in mediating plasticity, particularly in situ in the olfactory nerve in which they support the extension of olfactory sensory neuron (OSN) axons from the olfactory epithelium to the olfactory bulb (OB). OECs
David Campard et al.
Gastroenterology, 134(3), 833-848 (2008-02-05)
Umbilical cord matrix stem cells (UCMSCs) are able to differentiate into mesodermal and ectodermal lineages. The present study investigates the differentiation potential of human UCMSCs into hepatic lineage. We isolated human UCMSCs and characterized them in vitro by measuring their
X Li et al.
Neuroscience, 126(3), 611-630 (2004-06-09)
Gap junctions between glial cells in mammalian CNS are known to contain several connexins (Cx), including Cx26, Cx30 and Cx43 at astrocyte-to-astrocyte junctions, and Cx29 and Cx32 on the oligodendrocyte side of astrocyte-to-oligodendrocyte junctions. Recent reports indicating that oligodendrocytes also
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