Anti-KRIT-1 Antibody, clone 15

Cerebral cavernous malformation (CCM), a disease associated with defective endothelial junctions, results from autosomal dominant CCM1 mutations that cause a loss of KRIT-1 protein function. CCM1 has been identified as the KRIT-1 gene. The protein product, KRIT-1, also known as Krev interaction trapped protein 1, is a microtubule-associated protein involved in endothelial cell-cell and cell-extracellular matrix interactions. KRIT-1 binds to Rap1 that maintains the integrity of endothelial junctions. It is reported that KRIT-1 colocalizes and is physically associated with junctional proteins via its band 4.1/ezrin/radixin/moesin (FERM) domain, (Glading, 2007).

The antibody recognizes KRIT-1.

Epitope: FERM domain

GST-tagged recombinant protein corresponding to human KRIT-1 at and around the FERM domain.

Anti-KRIT-1 Antibody, clone 15 detects level of KRIT-1 & has been published & validated for use in IC, IP & WB.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected KRIT-1 in HeLa, A431, and HUVECs.

~ 84 kDa

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa, A431, and HUVECs.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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