MAB2259Z
Anti-Integrin β1 Antibody, activated, clone B44, azide free
clone B44, Chemicon®, from mouse
别名:
Ligand-inducedBindingSite, LIBS, CLIBS, MAB2259
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
B44, monoclonal
Species reactivity:
human
Application:
cell culture | mammalian
flow cytometry
immunohistochemistry
immunoprecipitation (IP)
western blot
flow cytometry
immunohistochemistry
immunoprecipitation (IP)
western blot
Technique(s):
cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
16
Uniprot accession no.:
产品名称
Anti-Integrin β1 Antibody, activated, clone B44, azide free, clone B44, Chemicon®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B44, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGB1(3688)
Application
This Anti-Integrin β1 Antibody, activated, clone B44, azide free is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.
Immunoprecipitation of detergent-solubilized (1 integrins: 2-4 μg/IP
Western blot: 10-15 μg/mL
Flow Cytometry: 10-15 μg/mL
Immunohistochemistry
Stimulation of (1-mediated adhesion: 10-20 μg/mL.
Optimal working dilutions must be determined by end user.
Western blot: 10-15 μg/mL
Flow Cytometry: 10-15 μg/mL
Immunohistochemistry
Stimulation of (1-mediated adhesion: 10-20 μg/mL.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Detects a cation- and ligand-induced binding site (LIBS) of integrin beta 1, which has been used in some systems as a reporter of integrin functionality.
General description
88 kDa
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. Liquid PBS, containing no preservatives.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
M Levite et al.
European journal of immunology, 31(12), 3504-3512 (2001-12-18)
Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major
Wan-Chun Chen et al.
American journal of physiology. Renal physiology, 307(6), F695-F707 (2014-07-25)
To explore whether matrix stiffness affects cell differentiation, proliferation, and transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in primary cultures of mouse proximal tubular epithelial cells (mPTECs), we used a soft matrix made from monomeric collagen type I-coated polyacrylamide gel
Cheng-Te Hsiao et al.
Oncotarget, 8(41), 70653-70668 (2017-10-21)
Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating
Izabela Staniszewska et al.
Journal of cell science, 121(Pt 4), 504-513 (2008-01-31)
The integrin alpha9beta1 is a multifunctional receptor that interacts with a variety of ligands including vascular cell adhesion molecule 1, tenascin C and osteopontin. We found that this integrin is a receptor for nerve growth factor (NGF) and two other
Weisheng Wang et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 41(11), 2723-2732 (2016-06-09)
Estradiol (E2) perfusion rapidly increases the strength of fast excitatory transmission and facilitates long-term potentiation in the hippocampus, two effects likely related to its memory-enhancing properties. Past studies showed that E2's facilitation of transmission involves activation of RhoA signaling leading
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