Anti-Tropomyosin Antibody, clone 15D12.2

Tropomyosins (Tms) are a family of >40 highly conserved protein isoforms resulting from alternative splicing of four different genes during development and that are expressed in numerous cell types. These proteins form coiled-coil heterodimers which bind to actin polymers along the α-helical grove. Tropomyosin’s function varies depending on cell type in which it is expressed. Within muscle, Tms play a role in the regulation of actin-myosin interplay for the purpose of mediating muscle contraction. In non-muscle cells, studies suggest a role in the stabilization of actin filaments, along with protecting actin filaments from the severing action of gelsolin and ADF/cofilin depolymerization. Tms also appear to have a role in the moderation of access to actin for other actin-binding proteins. Tropomyosin has an integral role in maintaining cardiovascular homeostasis. Down-regulation of Tms expression has been observed in tumor cells indicating a possible role for Tms as tumor suppressors.

The antibody recognizes the entire exon 9d of Tropomyosin.

Epitope: Exon 9d

Linear peptide corresponding to the entire exon 9d of rat Tropomyosin.

Detect Tropomyosin using this Anti-Tropomyosin Antibody, clone 15D12.2 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution from a representative lot detected Tropomyosin in human striated muscle tissue.

Research Category
Cell Structure

Metabolism

Research Sub Category
Cytoskeleton

Muscle Physiology

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of the antibody detected Tropomyosin in 10 µg of mouse brain tissue lysate.

~ 30-40 kDa observed MWs. Tm6: 40 kDa; Tm1: 38 kDa; Tm2: 36 kDa; Tm3: 34 kDa; Tm5: 30 kDa

Protein G

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse brain tissue lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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