生物来源
mouse
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
P2H3, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... SELE(6401)
一般描述
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
特异性
Human E-Selectin
免疫原
IL-1(1 activated HUVEC (human)
应用
Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.
Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
外形
Format: Purified
Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN3.
储存及稳定性
Maintain at 2-8°C for up to 12 months from date of receipt..
分析说明
Control
Ductal breast cancer or mormal colon
Ductal breast cancer or mormal colon
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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