Anti-Integrin β3 Antibody, clone B3A, azide free

Specific for human integrin beta 3 (other species not tested).

Human integrin alphaVbeta3 heterodimer isolated from placenta.

Anti-Integrin β3 Antibody, clone B3A, azide free detects level of Integrin β3 & has been published & validated for use in FC, FUNC & WB.

Flow cytometry

Function-blocking of integrin beta3-mediated adhesion to ECM proteins.

Western blot (reducing conditions) Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

110 kDa

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Positive Control: U251 or D54 human cell lines. Membrane preps are recommended for western blots.
Human endothelium cells (expression of integrin alpha V beta 3 may need to be upregulated by phorbol esters stimulation). Skin (Basement Membrane)

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.