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Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

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主要文件

安全信息

MAB1997

Sigma-Aldrich

Anti-Integrin beta 1 (CD29) Antibody

CHEMICON®, rat monoclonal, MB1.2

别名:

CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)

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25 μG
¥1,455.13
100 μG
¥6,364.93

¥1,455.13


预计发货时间2025年4月21日详情


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25 μG
¥1,455.13
100 μG
¥6,364.93

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

¥1,455.13


预计发货时间2025年4月21日详情


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产品名称

抗整合素β 1抗体,克隆MB1.2, clone MB1.2, Chemicon®, from rat

生物来源

rat

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

MB1.2, monoclonal

种属反应性

mouse, human

种属反应性(根据同源性预测)

rat

包装

antibody small pack of 25 μg

制造商/商品名称

Chemicon®

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1 of 4

此商品
MABT821MAB2247MABT199
biological source

rat

biological source

rat

biological source

mouse

biological source

mouse

clone

MB1.2, monoclonal

clone

mAb13, monoclonal

clone

12G10, monoclonal

clone

8E3, monoclonal

Gene Information

human ... ITGB1(3688)

Gene Information

human ... ITGB1(3688)

Gene Information

human ... ITGB1(3688)

Gene Information

human ... ITGB1(3688)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

technique(s)

flow cytometry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, inhibition assay: suitable

technique(s)

ELISA: suitable, cell culture | mammalian: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

activity assay: suitable, immunoprecipitation (IP): suitable, western blot: suitable

一般描述

整合素β-1(UniProt P05556;也被称为CD29、糖蛋白Iia、GPIIA、整合素VLA-4亚基β、极晚激活蛋白、β多肽)在人体中由ITGB1(也被称为FNRB、MDF2、MSK12、VLA-BETA、VLAB)基因(基因ID 3688)进行编码。 整合素β-1是一种130 kDa的跨膜糖蛋白,可与多种整合素α亚基(包括α1、α2、α3、α4、α5和α6)相互作用,形成与特定细胞外基质结合的功能性受体复合物蛋白。整合素受体可调节多种重要的生物学功能,包括胚胎发育、伤口修复、止血和防止程序性细胞死亡。它们还涉及异常的病理状态,例如肿瘤定向的血管生成、肿瘤生长和转移。这些异二聚体受体将细胞质肌动蛋白细胞骨架与细胞外基质和/或相邻细胞中存在的蛋白质进行桥接。整合素与细胞外基质之间的相互作用会导致信号转导途径的激活和基因表达的调节。

特异性

可与VLA(β1)整合素的β1亚基相互作用

免疫原

小鼠整合素β-1(Von Ballestrem,CG,et al.(1996).Hybridoma.15(2):125-132)。Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996).Hybridoma.15(2):125-132)。

应用

免疫沉淀
一个先前批次的产品已从106个细胞当量的裂解液中对VLA(β1)整合素进行了免疫沉淀:2 μg。

流式细胞术:
10 μg/mL先前批次的产品已被用于FC。

在冷冻组织切片中进行免疫组织化学分析:
使用20 μg/mL先前批次产品。由于VLA(β1)的组织分布广泛,通常能够观察到MAB1997与多种细胞类型发生结合。
其不会阻断VLA整合素介导的细胞对基质蛋白的粘附。通过MAB1997激活VLA(β1)整合素信号转导特性还尚未被表征。

蛋白质印迹:
10 µg/mL。对经过SDS变性和加热的样品有效。对还原后的样品无效。

最佳工作稀释度必须由最终用户确定。
抗整合素β1抗体,克隆MB1.2,是一种抗整合素β1抗体,可用于FC、IH、IP和&WB。

质量

已通过蛋白质印迹对C2C12裂解液进行了常规评估。

蛋白质印迹分析:
该批次的产品已以1:1000的稀释度在10 μg C2C12裂解物中检测到整合素β1。

目标描述

〜88 kDa(未糖基化)和〜130 kDa(糖基化)。

联系

替代品:04-1109

外形

形式:纯化
纯化的大鼠单克隆IgG2κ,溶于含0.02 M磷酸盐缓冲液(pH 7.6)、0.25 M NaCl和0.1%NaN3的缓冲液中

分析说明

对照
人OVCAR5细胞系全细胞裂解液

C2C12裂解液。

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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