产品名称
抗整合素α 1抗体，克隆FB12, clone FB12, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FB12, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ITGA1(3672)
Analysis Note
对照
微血管内皮
微血管内皮
Application
免疫组化：2 μg/mL，多聚甲醛固定。
免疫组化： 用丙酮固定1小时的胎盘组织的冷冻切片（7 µm）。抗体以1：10的稀释度进行使用（Sato et al.，2003）。
免疫细胞化学：通过0.5％PFA对分离的EVT细胞在4°C固定15分钟，然后在-20°C在丙酮中固定2分钟。 抗体以1：10的稀释度进行使用（Sato et al.，2003）。
免疫印迹：2 μg/mL
免疫沉淀：1 μg/mL
FACS分析：2μg/mL (Sato et al., 2005)。
ELISA：0.5 μg/mL
抑制结合层粘连蛋白、胶原蛋白IV 和纤连蛋白的、激活的人淋巴细胞(Fabbri et al., 1996): 1-5 μg/mL
最佳工作稀释度必须由最终用户进行确定。
免疫组化： 用丙酮固定1小时的胎盘组织的冷冻切片（7 µm）。抗体以1：10的稀释度进行使用（Sato et al.，2003）。
免疫细胞化学：通过0.5％PFA对分离的EVT细胞在4°C固定15分钟，然后在-20°C在丙酮中固定2分钟。 抗体以1：10的稀释度进行使用（Sato et al.，2003）。
免疫印迹：2 μg/mL
免疫沉淀：1 μg/mL
FACS分析：2μg/mL (Sato et al., 2005)。
ELISA：0.5 μg/mL
抑制结合层粘连蛋白、胶原蛋白IV 和纤连蛋白的、激活的人淋巴细胞(Fabbri et al., 1996): 1-5 μg/mL
最佳工作稀释度必须由最终用户进行确定。
该抗整联蛋白α1 抗体（克隆FB12）经验证可用于ELISA、FC、 IC、IH、IP、FUNC和WB检测整联蛋白α1。
Biochem/physiol Actions
与人α-1整联蛋白（CD49a）1的I 结构域（Val151 - Ala364）反应。从人淋巴细胞裂解液中沉淀相对分子质量180 kDa、pI 5.9-6.0（α链）的蛋白和相对分子质量115 kDa, pI 4.5-5.0（β链）的蛋白（Zocchi et al., 1991）。
General description
130 kDa
Other Notes
浓度：请参考批次特异性浓缩物的检验报告。
Physical form
形式：纯化
溶于0.02 M PB（pH值7.6）、含0.1%叠氮化钠 0.25 M NaCl的纯化免疫球蛋白。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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