跳转至内容
Merck
CN
所有图片(2)

主要文件

MAB1973

Sigma-Aldrich

抗整合素α 1抗体,克隆FB12

clone FB12, Chemicon®, from mouse

别名:

CD49a

登录查看公司和协议定价


About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

FB12, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... ITGA1(3672)

特异性

与人α-1整联蛋白(CD49a)1的I 结构域(Val151 - Ala364)反应。从人淋巴细胞裂解液中沉淀相对分子质量180 kDa、pI 5.9-6.0(α链)的蛋白和相对分子质量115 kDa, pI 4.5-5.0(β链)的蛋白(Zocchi et al., 1991)。

应用

免疫组化:2 μg/mL,多聚甲醛固定。
免疫组化: 用丙酮固定1小时的胎盘组织的冷冻切片(7 µm)。抗体以1:10的稀释度进行使用(Sato et al.,2003)。
免疫细胞化学:通过0.5%PFA对分离的EVT细胞在4°C固定15分钟,然后在-20°C在丙酮中固定2分钟。 抗体以1:10的稀释度进行使用(Sato et al.,2003)。
免疫印迹:2 μg/mL

免疫沉淀:1 μg/mL

FACS分析:2μg/mL (Sato et al., 2005)。

ELISA:0.5 μg/mL

抑制结合层粘连蛋白、胶原蛋白IV 和纤连蛋白的、激活的人淋巴细胞(Fabbri et al., 1996): 1-5 μg/mL

最佳工作稀释度必须由最终用户进行确定。
该抗整联蛋白α1 抗体(克隆FB12)经验证可用于ELISA、FC、 IC、IH、IP、FUNC和WB检测整联蛋白α1。

目标描述

130 kDa

外形

形式:纯化
溶于0.02 M PB(pH值7.6)、含0.1%叠氮化钠 0.25 M NaCl的纯化免疫球蛋白。

分析说明

对照
微血管内皮

其他说明

浓度:请参考批次特异性浓缩物的检验报告。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

未找到合适的产品?  

试试我们的产品选型工具.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Leo K Iwai et al.
The Biochemical journal, 454(3), 501-513 (2013-07-05)
Collagen is an important extracellular matrix component that directs many fundamental cellular processes including differentiation, proliferation and motility. The signalling networks driving these processes are propagated by collagen receptors such as the β1 integrins and the DDRs (discoidin domain receptors).
Katarzyna Biadasiewicz et al.
Endocrinology, 152(4), 1458-1469 (2011-02-10)
The basic helix-span-helix transcription factor activating protein (AP)-2α is critically involved in cell-specific hormone expression of syncytializing human trophoblasts. Its role in invasive trophoblast differentiation, however, remains largely elusive. Using RT-PCR, Western blotting, and immunofluorescence of first-trimester placentae, we here
Huifang Xu et al.
PloS one, 7(12), e52209-e52209 (2013-01-04)
The discoidin domain receptors, DDR1 and DDR2, are receptor tyrosine kinases that bind to and are activated by collagens. Similar to collagen-binding β1 integrins, the DDRs bind to specific motifs within the collagen triple helix. However, these two types of
B K Pilcher et al.
The Journal of cell biology, 137(6), 1445-1457 (1997-06-16)
We have shown in a variety of human wounds that collagenase-1 (MMP-1), a matrix metalloproteinase that cleaves fibrillar type I collagen, is invariably expressed by basal keratinocytes migrating across the dermal matrix. Furthermore, we have demonstrated that MMP-1 expression is
Steve Gendron et al.
The Journal of biological chemistry, 278(49), 48633-48643 (2003-09-19)
The mechanisms by which T lymphocytes escape apoptosis during their activation are still poorly defined. In this study, we elucidated the intracellular signaling pathways through which beta1 integrins modulate Fas-mediated apoptosis in T lymphocytes. In experiments done in Jurkat T

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门