推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
FB12, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ITGA1(3672)
特异性
与人α-1整联蛋白(CD49a)1的I 结构域(Val151 - Ala364)反应。从人淋巴细胞裂解液中沉淀相对分子质量180 kDa、pI 5.9-6.0(α链)的蛋白和相对分子质量115 kDa, pI 4.5-5.0(β链)的蛋白(Zocchi et al., 1991)。
应用
免疫组化:2 μg/mL,多聚甲醛固定。
免疫组化: 用丙酮固定1小时的胎盘组织的冷冻切片(7 µm)。抗体以1:10的稀释度进行使用(Sato et al.,2003)。
免疫细胞化学:通过0.5%PFA对分离的EVT细胞在4°C固定15分钟,然后在-20°C在丙酮中固定2分钟。 抗体以1:10的稀释度进行使用(Sato et al.,2003)。
免疫印迹:2 μg/mL
免疫沉淀:1 μg/mL
FACS分析:2μg/mL (Sato et al., 2005)。
ELISA:0.5 μg/mL
抑制结合层粘连蛋白、胶原蛋白IV 和纤连蛋白的、激活的人淋巴细胞(Fabbri et al., 1996): 1-5 μg/mL
最佳工作稀释度必须由最终用户进行确定。
免疫组化: 用丙酮固定1小时的胎盘组织的冷冻切片(7 µm)。抗体以1:10的稀释度进行使用(Sato et al.,2003)。
免疫细胞化学:通过0.5%PFA对分离的EVT细胞在4°C固定15分钟,然后在-20°C在丙酮中固定2分钟。 抗体以1:10的稀释度进行使用(Sato et al.,2003)。
免疫印迹:2 μg/mL
免疫沉淀:1 μg/mL
FACS分析:2μg/mL (Sato et al., 2005)。
ELISA:0.5 μg/mL
抑制结合层粘连蛋白、胶原蛋白IV 和纤连蛋白的、激活的人淋巴细胞(Fabbri et al., 1996): 1-5 μg/mL
最佳工作稀释度必须由最终用户进行确定。
该抗整联蛋白α1 抗体(克隆FB12)经验证可用于ELISA、FC、 IC、IH、IP、FUNC和WB检测整联蛋白α1。
目标描述
130 kDa
外形
形式:纯化
溶于0.02 M PB(pH值7.6)、含0.1%叠氮化钠 0.25 M NaCl的纯化免疫球蛋白。
分析说明
对照
微血管内皮
微血管内皮
其他说明
浓度:请参考批次特异性浓缩物的检验报告。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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