Anti-Integrin β4 Antibody

Integrin β4 (UniProt: P16144; also known as GP150, CD104) is encoded by the ITGB4 gene (Gene ID: 3691) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrins bind to their ligands with low affinity and this binding occurs only when a certain minimum number of integrins are present at specific points known as focal contacts. Integrin β4 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 28-710), a transmembrane domain (aa 711-733), and a cytoplasmic domain (aa 734-1822). It contains four fibronectin type III domains. Integrin β4 heterodimerizes with a6 that serves as a receptor for laminin and plays a critical structural role in the hemidesmosome of epithelial cells. It undergoes palmitoylation by DHHC3 at several cysteines of the membrane-proximal region that enhances its stability and surface expression. Palmitoylation also promotes its secondary association with tetraspanins.

Clone 3E1 is a mouse monoclonal antibody that detects Integrin β4. It targets an epitope within the extracellular domain.

Human fetal cell membranes.

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Integrin β4 in A431 cells.

Tested Applications

Flow Cytometry Analysis: 1 βg from a representative lot detected Integrin β4 in one million A431 cells.

Flow Cytometry Analysis: A representative lot detected Integrin β4 in Flow Cytometry applications (Vijayakumar, S., et. al. (2008). J. Am. Soc. Nephrol.19(6):1079-91; Hauptmann, S., et. al. (1995). Int. J. Cancer. 61(6):819-25).

Immunohistochemistry Applications: A representative lot detected Integrin β4 in Immunohistochemistry applications ( Hauptmann, S., et. al. (1995). Int. J. Cancer. 61(6):819-25).

Immunofluorescence Analysis: A representative lot detected Integrin β4 in Immunofluorescence applications (Swanner, J., et. al. (2019). FASEB BioAdv. 1(10):639-660).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Integrin β4, clone 3E1, Cat. No. MAB1964-I, is a mouse monoclonal antibody that detects Integrin β4 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at +2°C to +8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.