推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
9D2.2, monoclonal
种属反应性
mouse
制造商/商品名称
Chemicon®
技术
ELISA: suitable
western blot: suitable
同位素/亚型
IgG2b
适用性
not suitable for immunohistochemistry
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ADAM2(2515)
特异性
通过免疫印迹识别小鼠受精蛋白β，在46-48 kDa范围内进行条带染色（多条带是由于可变蛋白质糖基化）。
免疫原
通过在SF9细胞中表达杆状病毒产生重组小鼠受精蛋白。
应用
研究子类别
ECM蛋白
MMP & TIMP
ECM蛋白
MMP & TIMP
研究类别
细胞结构
细胞结构
蛋白质印迹：1:500 - 1:1,000稀释液可检测精子裂解物，使用标准碱性磷酸酶与NBT/BCIP检测。 化学发光检测允许更高的稀释度。
对免疫组织化学无效。
最佳工作稀释度必须由最终用户进行确定。
对免疫组织化学无效。
最佳工作稀释度必须由最终用户进行确定。
该抗-ADAM2抗体，克隆9D2.2，经验证可用于ELISA、WB中ADAM2的检测。
外形
形式：纯化
液体形式，1 mg/mL，溶于0.02M磷酸盐缓冲液，pH 7.6，含0.25M NaCl和0.1%叠氮化钠。
储存及稳定性
以未稀释的等分形式可在2-8°C冷藏保存长达12个月。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Daiji Kiyozumi et al.
Biology of reproduction, 109(4), 474-481 (2023-08-02)
The mammalian epididymis is the organ for functional sperm maturation. In rodents, the initial segment, the most proximal region of the epididymis, plays a critical role in sperm maturation. The luminal epithelial differentiation and the following gene expression of the
Ryo Yamaguchi et al.
Biology of reproduction, 75(5), 760-766 (2006-07-28)
Male mice deficient for the calmegin (Clgn) or the angiotensin-converting enzyme (Ace) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN is a molecular chaperone for membrane transport of target
Kathryn K Stein et al.
Biology of reproduction, 73(5), 1032-1038 (2005-07-15)
Adam2-null and Adam3-null male mice exhibit reduced levels of one or more ADAM proteins on mature sperm, in addition to the loss of the genetically targeted protein. ADAM protein loss was believed to occur posttranslationally, although the timing of loss
Yasutaka Ueda et al.
The Journal of biological chemistry, 282(42), 30373-30380 (2007-08-23)
A palmitate linked to the inositol in glycosylphosphatidylinositol (GPI) is removed in the endoplasmic reticulum immediately after the conjugation of GPI with proteins in most cells. Previously, we identified PGAP1 (post GPI attachment to proteins 1) as a GPI inositoldeacylase
Ryo Yamaguchi et al.
Genes to cells : devoted to molecular & cellular mechanisms, 13(8), 851-861 (2008-09-11)
CD52 is a glycosylphosphatidylinositol (GPI)-anchored antigen expressed on lymphocytes and in epididymal epithelial cells. CD52 is also known as "maturation-associated sperm antigen" but its function is unknown. We therefore generated Cd52 disrupted mice. The resulting Cd52 null mice were healthy
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