biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9D2.2, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
isotype
IgG2b
suitability
not suitable for immunohistochemistry
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ADAM2(2515)
Immunogen
通过在SF9细胞中表达杆状病毒产生重组小鼠受精蛋白。
Application
研究子类别
ECM蛋白
MMP & TIMP
ECM蛋白
MMP & TIMP
研究类别
细胞结构
细胞结构
蛋白质印迹：1:500 - 1:1,000稀释液可检测精子裂解物，使用标准碱性磷酸酶与NBT/BCIP检测。 化学发光检测允许更高的稀释度。
对免疫组织化学无效。
最佳工作稀释度必须由最终用户进行确定。
对免疫组织化学无效。
最佳工作稀释度必须由最终用户进行确定。
该抗-ADAM2抗体，克隆9D2.2，经验证可用于ELISA、WB中ADAM2的检测。
Biochem/physiol Actions
通过免疫印迹识别小鼠受精蛋白β，在46-48 kDa范围内进行条带染色（多条带是由于可变蛋白质糖基化）。
Physical form
形式：纯化
液体形式，1 mg/mL，溶于0.02M磷酸盐缓冲液，pH 7.6，含0.25M NaCl和0.1%叠氮化钠。
Preparation Note
以未稀释的等分形式可在2-8°C冷藏保存长达12个月。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Daiji Kiyozumi et al.
Biology of reproduction, 109(4), 474-481 (2023-08-02)
The mammalian epididymis is the organ for functional sperm maturation. In rodents, the initial segment, the most proximal region of the epididymis, plays a critical role in sperm maturation. The luminal epithelial differentiation and the following gene expression of the
Ryo Yamaguchi et al.
Biology of reproduction, 75(5), 760-766 (2006-07-28)
Male mice deficient for the calmegin (Clgn) or the angiotensin-converting enzyme (Ace) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN is a molecular chaperone for membrane transport of target
Ryo Yamaguchi et al.
Genes to cells : devoted to molecular & cellular mechanisms, 13(8), 851-861 (2008-09-11)
CD52 is a glycosylphosphatidylinositol (GPI)-anchored antigen expressed on lymphocytes and in epididymal epithelial cells. CD52 is also known as "maturation-associated sperm antigen" but its function is unknown. We therefore generated Cd52 disrupted mice. The resulting Cd52 null mice were healthy
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB19292 | 04053252265778 |