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生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
5H2, monoclonal
种属反应性
human, rabbit, monkey
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... LAMA2(3908)
rhesus monkey ... Lama2(715394)
特异性
Reacts with the 80 kDa fragment of the M-chain of human merosin.
SPECIES REACTIVITIES:
Cross reacts with monkey and rabbit merosin.
SPECIES REACTIVITIES:
Cross reacts with monkey and rabbit merosin.
免疫原
Purified human merosin.
应用
Anti-Laminin α2 Antibody, clone 5H2, Azide Free detects level of Laminin α2 & has been published & validated for use in ELISA, IH, IP & WB.
ELISA: 50% maximal binding to human merosin at 1:50,000.
Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence
Affinity chromatography
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:5,000 for staining of 8 mm acetone-fixed cryostat muscle sections, prior to detection with a peroxidase-conjugated secondary antibody.
Immunofluorescence
Affinity chromatography
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
外形
Format: Purified
储存及稳定性
Maintain between 2 and 8°C for up to 6 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Corinna Preuße et al.
Acta neuropathologica communications, 4(1), 45-45 (2016-04-29)
Dermatomyositis (DM) can occur in both adults and juveniles with considerable clinical differences. The links between immune-mediated mechanisms and vasculopathy with respect to development of perifascicular pathology are incompletely understood. We investigated skeletal muscle from newly diagnosed, treatment-naïve juvenile (jDM)
Elizabeth I Tang et al.
Endocrinology, 163(11) (2022-08-17)
Microtubule affinity-regulating kinases (MARKs) are nonreceptor Ser/Thr protein kinases known to regulate cell polarity and microtubule dynamics in Caenorhabditis elegans, Drosophila, invertebrates, vertebrates, and mammals. An earlier study has shown that MARK4 is present at the ectoplasmic specialization and blood-testis
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