Anti-Aggrecan Core Protein Antibody, clone Cat-315

Aggrecan Core Protein (UniProt: A0A5F5XE37; also known as Anti-Chondroitin Sulfate Proteoglycan core protein, CSPG1) is encoded by the ACAN gene (Gene ID: 101084542) in cat. Chondroitin sulfate proteoglycans (CSPGs) are a family of large extracellular matrix molecules, composed of a central core protein to which a varying number of glycosaminoglycan (GAG) side chains are attached. They are widely expressed in the mammalian central nervous system. CSPG1 consists of a protein core and a chondroitin sulfate side chain. CSPG1 lacks a transmembrane domain and the entire molecule is extracellular and, therefore, is considered a constituent of brain extracellular matrix. It is synthesized with a signal peptide (aa 1-16, which is subsequently cleaved off to generate the mature form. CSPG1 plays a role in neural development, plasticity, and post-injury response. CSPGs are involved in cell adhesion, cell growth, receptor binding, and cell migration. They are reported to inhibit axon regeneration after spinal cord injury and contribute to glial scar formation, which blocks new axons from growing into the injury site. During development CSPGs are reported to act as guidance cues for developing growth cones. In the adult, they stabilize synaptic connections and prevent aberrant axonal sprouting. Clone Cat-315 is shown to recognize a chondroitin sulfate proteoglycan (CSPG) expressed on the surface of subsets of neurons in many areas of the mammalian CNS. (Ref.: Siebert, JR., and Osterhout, DJ. (2011). J. Neurochem. 119(1); 186-188; Mathews, RT., et al. (2002). J. Neurosci. 22(17); 7536-7547; Lander, C., et al. (1998). J. Neurosci. 18(1); 174-183).

Clone Cat-315 is a mouse monoclonal antibody that detects Aggrecan core protein (CSPG1). It detects an epitope on the extracellular region.

Feline brain proteoglycans.

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aggrecan Core Protein in Rat brain tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aggrecan Core Protein in cortex tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:150 dilution from a representative lot detected Aggrecan Core Protein in rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aggrecan Core Protein in Rat e18 cortical cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aggrecan Core Protein in Immunoprecipitation applications (Lander, C., et. al. (1998). J Neurosci. 18(1):174-83).

Immunohistochemistry Applications: A representative lot detected Aggrecan Core Protein in Immunohistochemistry applications (Grant, E., et. al. (2016). Cereb Cortex. 26(3):1336-1348; Lander, C., et. al. (1998). J Neurosci. 18(1):174-83; Quattromani, M.J., et. al. (2018). Mol Neurobiol. 55(3):2196-2213).

Immunofluorescence Analysis: A representative lot detected Aggrecan Core Protein in Immunofluorescence applications (Quattromani, M.J., et. al. (2018). Mol Neurobiol. 55(3):2196-2213).

Western Blotting Analysis: A representative lot detected Aggrecan Core Protein in Western Blotting applications (Choi, B.R., et. al. (2020). Cell Rep. 31(5):107540; Quattromani, M.J., et. al. (2018). Mol Neurobiol. 55(3):2196-2213; Lander, C., et. al. (1998). J Neurosci. 18(1):174-83).

Immunocytochemistry Analysis: A representative lot detected Aggrecan Core Protein in Immunocytochemistry applications (Lander, C., et. al. (1998). J Neurosci. 18(1):174-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Aggrecan Core Protein, clone Cat-315, Cat. No. MAB1581-I, is a mouse monoclonal antibody that detects Aggrecan Core Protein and is used in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Purified mouse monoclonal antibody IgM in PBS without azide.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.