Anti-TIMP-2 Antibody, clone 2TMP05

MAB13446 recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinase-2 (TIMP-2). TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. CHEMICON MAB13446 may be used to evaluate the invasive nature of some tumor types and determine the tissue distribution of TIMPs in other tumors such as adenomas and also its normal tissue.

CELLULAR LOCALIZATION:

Cytoplasmic

A synthetic peptide from the N-terminal region of human TIMP-2 protein.

Detect TIMP-2 using this Anti-TIMP-2 Antibody, clone 2TMP05 validated for use in WB, IH(P).

Immunohistology (formalin/paraffin only): 2-4μg/ml 30 min at RT.

Western blotting: 1μg/ml for 2 hrs at RT.

(Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min).

The optimal dilution for a specific application under a given set of experimental conditions should be determined by the investigator.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Replaces: MAB13448

Format: Purified

Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Antibody with sodium azide is stable for 12 months after date of receipt when stored at 2-8°C.

Control
POSITIVE CONTROL:

Pancreatic or colon carcinoma

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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