推荐产品
生物来源
mouse
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
7C7, monoclonal
种属反应性
adenovirus-infected cells, virus
包装
antibody small pack of 25 μL
技术
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG2bκ
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
Capsid protein VP1 (UniProt: Q6JC40) is encoded by the Cap gene in Adeno-associated virus 9 (AAV9). Adeno-associated viruses (AAV) are small non-enveloped DNA viruses that can serve as promising viral vectors for gene delivery, but can also elicits cellular and humoral immune responses. AAVs are reported to be nonpathogenic in humans and animals and display low immunogenicity. Following their attachment to cell surface receptor they are internalized via endocytosis and their intracellular trafficking is a rate-limiting, slow and inefficient process. AAVs contain an icosahedral capsid, which is 22 to 26 nm in diameter. They are shown to have a single-stranded DNA genome of approximately 4.7 kilobases that is flanked by two T-shaped inverted terminal repeats (ITRs). The 7C7 an epitope-tag antibody recognizes a basic ten amino acid epitope (QPAKKRLNFGQTGD) with high specificity. The basic nature of the BASE-tag epitope sequence distinguishes it from other commonly used epitope tags, such as Myc, EQKLISEEDL, FLAG, DYKDDDDK, HA, YPYDVPDYA, V5 etc. Clone 7C7 recognizes capsid proteins VP1 and VP2, but not VP3. Based on sequence identity of the epitope sequence, this antibody is expected to recognize VP1 and VP2 of AAV serotypes 1,2,3,6,7,8, and 9. (Ref.: Nonnenmacher, M., and Weber, T (2012). Gene Ther. 19(6); 649-658).
特异性
Clone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
免疫原
Empty Adeno-associated virus 9 (AAV9) Capsids
应用
Anti-BASE-tag, clone 7C7, Cat. No. MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence.
Immunoprecipitation: A representative lot immunoprecipitated EGFP-BASE-tag-Actin in HEK293T cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
Research Category
Inflammation & Immunology
Inflammation & Immunology
质量
Evaluated by Western Blotting in HEK293 cells transfected with GFP-7C7-Actin.
Western Blotting Analysis: A 1:500 dilution of this antibody detected BASE-tag in HEK293 cells transfected with GFP-7C7-Actin.
Western Blotting Analysis: A 1:500 dilution of this antibody detected BASE-tag in HEK293 cells transfected with GFP-7C7-Actin.
外形
Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品?
试试我们的产品选型工具.
法规信息
新产品
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门