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MAB1285

Sigma-Aldrich

抗HLA-B27抗体,克隆HLA.ABC.M3

clone HLA.ABC.m3, Chemicon®, from mouse

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别名:
Anti-AS, Anti-B-4901, Anti-HLAB
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.43

生物来源

mouse

质量水平

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

HLA.ABC.m3, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

flow cytometry: suitable
immunocytochemistry: suitable

同位素/亚型

IgG2a

NCBI登记号

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... HLA-B(3106)

一般描述

之前分配的蛋白标识符P03989已合并到P01889中。完整的详细资料见于UniProt数据库。

特异性

该抗体与HLA-B27抗原牢固结合。它与HLA-B7抗原的结合强度也较低。通过斯卡查德分析进行的亲和力研究表明,该抗体对HLA-B27(9.7 x108M-1)的亲和力高于对HLA-B7

(9.5 x107M-1)的亲和力。

细胞反应性:

HLA-B27淋巴细胞染色的流式细胞术分析得出以下结果:



受试者 荧光强度

杂合HLA-B27+ 强

纯合HLA-B7+ 强

杂合HLA-B7+ 微弱的

非B27,非B7 阴性

应用

该抗HLA-B27抗体(克隆HLA.ABC.m3)经验证可用于FC,IC检测HLA-B27。
该抗体可用于强直性脊柱炎和前葡萄膜炎等疾病中HLA-B27的检查。

建议使用 稀释度

1.流式细胞术和间接免疫荧光1:60~1:120

用等渗缓冲液稀释。在100μl缓冲液中,每1 x 10E6外周血单核细胞(PBMC)使用50 μl稀释的抗体。

2.细胞毒性试验1:25。

外形

形式:纯化
抗体以含有0.2%牛血清白蛋白和0.1%叠氮化钠的磷酸盐缓冲盐水(pH 7.4)提供。 通过流式细胞仪检测每个批次的特性。

储存及稳定性

保存于2-8°C。对于延长的周期,以未稀释的等分试样储存在-20°C以下。应避免反复冻/融循环。

警告:单克隆试剂溶液含有0.1%叠氮化钠作为防腐剂。由于该物料在管道中积聚会产生潜在危害,因此应将废试剂与大量水一起处置。

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

WGK 2


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