MAB1273

抗线粒体抗体，完整线粒体表面，克隆113-1

Name: 抗线粒体抗体，完整线粒体表面，克隆113-1
Brand: MM

clone 113-1, Chemicon^®, from mouse

别名:

线粒体抗体

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

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产品名称

抗线粒体抗体，完整线粒体表面，克隆113-1, clone 113-1, Chemicon^®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

113-1, monoclonal

species reactivity

human

should not react with

mouse, rat

manufacturer/tradename

Chemicon^®

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

input

sample type neural stem cell(s)

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Analysis Note

对照
COS7细胞。

通过免疫组化对心室细胞进行常规评估。

免疫组织化学（石蜡）分析：心室细胞上的
线粒体（目录号MAB1273）染色。用EDTA、pH值8.0预处理的组织。将该批次抗体使用带有HRP-DAB的IHC-SelectDetection稀释至1：80。免疫反应性可见细胞核周围的圆形颗粒层。
EDTA缓冲液pH值8.0进行最佳染色，表位修复：心室上皮

Application

免疫组化：
使用先前批次的1:100稀释液。与有机固定剂（丙酮、甲醇等）配合使用效果最好。可以使用2%PFA 10-15分钟，但更强烈地使用福尔马林或4%PFA限制染色。
该抗体已用于通过与磁珠结合从活细胞的冻融裂解物中分离完整的线粒体{http：//www.dynalbiotech.com}。

石蜡包埋组织的免疫组织化学：
需要柠檬酸/微波抗原修复；推荐使用增强的ABC或扩增检测系统。
使用EDTA缓冲液pH值8.0进行最佳染色，表位修复：心室上皮

该抗体已被用于通过与磁珠结合从活细胞的冻融裂解物中分离完整的线粒体{http：//www.dynalbiotech.com}。

间接免疫荧光：
使用先前批次的1：10-1：50稀释液，每个玻片孔30-100 μL。

MAB1273通过免疫沉淀识别65 kD蛋白。

最佳工作稀释度和方案必须由最终用户确定。

抗线粒体抗体，完整线粒体的表面，克隆113-1是针对线粒体的抗体，用于IF，IH，IH（P）&IP。

Biochem/physiol Actions

与其他物种的反应性尚未确定

在全部人细胞类型上进行线粒体染色，并且不与大鼠和小鼠组织发生交叉反应。染色模式最好描述为成纤维细胞中的意大利面条状染色模式和淋巴样细胞中的核周大斑点模式。
MAB1273通过免疫沉淀识别65 kD蛋白。抗体在人细胞的细胞质中产生细线状的“意大利面条状”染色模式。

General description

65kda

该抗体可识别人细胞中线粒体的60 kDa非糖基化蛋白组分。它可用于染色细胞、组织或生化制剂中的线粒体，并可用作亚细胞组分中线粒体的标志物。

Immunogen

表位：完整线粒体表面

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

在含PBS液体的缓冲液中纯化的小鼠单克隆IgG1。不含防腐剂。浓缩的杂交瘤上清液。

形式：纯化

Preparation Note

自收到之日起，以未稀释的等分试样可于-20°C条件下稳定保存6个月。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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Novel metastatic models of esophageal adenocarcinoma derived from FLO-1 cells highlight the importance of E-cadherin in cancer metastasis.

David S Liu et al.

Oncotarget, 7(50), 83342-83358 (2016-11-20)

There is currently a paucity of preclinical models available to study the metastatic process in esophageal cancer. Here we report FLO-1, and its isogenic derivative FLO-1LM, as two spontaneously metastatic cell line models of human esophageal adenocarcinoma. We show that

Matrix remodeling stimulates stromal autophagy, "fueling" cancer cell mitochondrial metabolism and metastasis.

Remedios Castello-Cros et al.

Cell cycle (Georgetown, Tex.), 10(12), 2021-2034 (2011-06-08)

We have previously demonstrated that loss of stromal caveolin-1 (Cav-1) in cancer-associated fibroblasts is a strong and independent predictor of poor clinical outcome in human breast cancer patients. However, the signaling mechanism(s) by which Cav-1 downregulation leads to this tumor-promoting

Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness.

Hongshuai Li et al.

PloS one, 8(6), e64923-e64923 (2013-06-14)

Human muscle-derived progenitor cells (hMDPCs) offer great promise for muscle cell-based regenerative medicine; however, prolonged ex-vivo expansion using animal sera is necessary to acquire sufficient cells for transplantation. Due to the risks associated with the use of animal sera, the

Enteric neurons from Parkinson's disease patients display ex vivo aberrations in mitochondrial structure.

A S Baumuratov et al.

Scientific reports, 6, 33117-33117 (2016-09-15)

Based on autopsy material mitochondrial dysfunction has been proposed being part of the pathophysiological cascade of Parkinson's disease (PD). However, in living patients, evidence for such dysfunction is scarce. As the disease presumably starts at the enteric level, we studied

Vascular endothelial growth factor-D over-expressing tumor cells induce differential effects on uterine vasculature in a mouse model of endometrial cancer.

Jane E Girling et al.

Reproductive biology and endocrinology : RB&E, 8, 84-84 (2010-07-10)

It has been hypothesised that increased VEGF-D expression may be an independent prognostic factor for endometrial cancer progression and lymph node metastasis; however, the mechanism by which VEGF-D may promote disease progression in women with endometrial cancer has not been

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抗线粒体抗体，完整线粒体表面，克隆113-1

clone 113-1, Chemicon®, from mouse

选择尺寸

关于此项目

主要文件

属性

产品名称

biological source

antibody form

antibody product type

clone

species reactivity

should not react with

manufacturer/tradename

technique(s)

input

isotype

shipped in

target post-translational modification

Quality Level

相关类别

说明

Analysis Note

Application

Biochem/physiol Actions

General description

Immunogen

Other Notes

Physical form

Preparation Note

Legal Information

安全信息

存储类别

wgk

flash_point_f

flash_point_c

文件

分析证书(COA)

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clone 113-1, Chemicon^®, from mouse