推荐产品
生物来源
mouse
质量水平
偶联物
alkaline phosphatase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
G3, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TPSAB1(7177)
特异性
Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.
应用
Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase is an antibody against Tryptase for use in WB, IH.
Immunoblotting & immunohistochemistry at 1:200-1:1,000. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovsky′s reagent [Lab. Invest. (1988). 58:682-691].
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
外形
In 0.05M Tris + 0.1M NaCl + 0.001M MgCl2 with 0.1% NaN3, pH=8.0.
储存及稳定性
Maintain at +2-8°C in undiluted aliquots for up to 6 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Premkumar Siddhuraj et al.
Cells, 10(2) (2021-02-07)
The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on
David Lamb et al.
Respiratory research, 22(1), 158-158 (2021-05-24)
RORγt is a transcription factor that enables elaboration of Th17-associated cytokines (including IL-17 and IL-22) and is proposed as a pharmacological target for severe asthma. IL-17 immunohistochemistry was performed in severe asthma bronchial biopsies (specificity confirmed with in situ hybridization).
Premkumar Siddhuraj et al.
Frontiers in immunology, 13, 924244-924244 (2022-08-20)
The mast cell-specific metalloprotease CPA3 has been given important roles in lung tissue homeostasis and disease pathogenesis. However, the dynamics and spatial distribution of mast cell CPA3 expression in lung diseases remain unknown. Using a histology-based approach for quantitative spatial
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