MAB1120
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31
clone 24-31, Chemicon®, from mouse
别名:
IGF-IR, CD221
产品名称
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31, clone 24-31, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
24-31, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IGF1R(3480)
Application
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.
Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.
Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.
Optimal working dilutions must be determined by end user.
Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.
Biochem/physiol Actions
Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.
Weakly reacts with Rabbit.
Weakly reacts with Rabbit.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.
Physical form
Format: Purified
Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
[Articles of the Hungarian Medical Journal from September, 2006].
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