推荐产品
一般描述
来自维多利亚多管发光水母(Aequorea victoria)的绿色荧光蛋白(GFP)可用作荧光指示剂,用于监测各种细胞系统(包括活生物体和固定组织)中的基因表达。与其他生物发光报告基因不同,GFP在没有底物、辅因子或其他内在或外在蛋白质的情况下发出荧光。纯化的GFP是一种27 kDa的单体,由238个氨基酸组成,当被蓝色或紫外线激发时会发出绿色荧光(最大发射波长为509 nm)。
特异性
与大多数含有GFP标签的常见脊椎动物发生反应
抗体与28 kDa GFP蛋白反应。
免疫原
全长 GFP 蛋白
表位:全长
应用
使用经验证可用于WB的该抗-GFP抗体(克隆3F8.2)检测GFP。
研究子类别
表位标签
表位标签
研究范畴
二抗&对照抗体
二抗&对照抗体
蛋白质印迹分析:
0.125 μg/mL - 1 μg/mLl该抗体可在10 ng的GFP 重组蛋白中检测到GFP(货号14-392) 。
0.125 μg/mL - 1 μg/mLl该抗体可在10 ng的GFP 重组蛋白中检测到GFP(货号14-392) 。
质量
已通过蛋白质印迹对GFP重组蛋白进行常规评估。
目标描述
28 kDa
外形
形式:纯化
纯化小鼠免疫球蛋白,溶于含0.05%叠氮化钠的PBS缓冲液。
蛋白G纯化
储存及稳定性
自收到之日起,在2-8°C以未稀释的等分试样可保存1年。避免反复冻融循环,以免损坏IgG和影响产品性能。
分析说明
对照
蛋白质印迹法:GFP 重组蛋白
蛋白质印迹法:GFP 重组蛋白
其他说明
浓度:请参考批次特异性浓缩物的检验报告。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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