推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
20, monoclonal
种属反应性
human
浓度
1.0 mg/mL
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
链霉亲和素结合肽标签是一种有用且用途广泛的肽,可以插入表达载体中,以便于重组蛋白的检测和纯化。SBP标签的序列长38个氨基酸,对链霉亲和素具有纳摩尔规模结合亲和力,平衡常数为2.5 nM。
特异性
证明与表达SBP标签的细胞反应。
该抗体识别SBP标签。
免疫原
对应于SBP标签的线性肽。
表位:未知
应用
免疫荧光分析: 先前批次已被独立实验室用于IF。(Hudson, D., et al. (2008).MBC. 19(7):3070-3079.)
研究子类别
表位标签
表位标签
研究类别
表位标签 & 一般用途
表位标签 & 一般用途
质量
已通过蛋白质印迹在pTrAP-GFP转染的Hek293裂解物中进行了评估。
蛋白质印迹分析:0.1 ng/ml的该抗体在10 µg pTrAP-GFP转染的Hek293裂解物上检测到SBP标签。
蛋白质印迹分析:0.1 ng/ml的该抗体在10 µg pTrAP-GFP转染的Hek293裂解物上检测到SBP标签。
目标描述
蛋白表达是包含组氨酸标签,S标签,链霉亲和素结合肽(SBP)标签和GFP的pTrAP-GFP质粒。表达的蛋白约为37kDa
外形
在含 0.1 M Tris-甘氨酸(pH 7.4,150 mM NaCl) 和 0.05% 叠氮化钠的缓冲液中的纯化的小鼠单克隆 IgG1κ。
形式:纯化
纯化的蛋白G
储存及稳定性
自收到之日起,在 2-8°C 条件下可稳定保存1年
分析说明
对照
pTrAP-GFP转染的Hek293裂解物
pTrAP-GFP转染的Hek293裂解物
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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