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主要文件

MAB10764

Sigma-Aldrich

抗SBP标签抗体,克隆20|MAB10764

clone 20, 1.0 mg/mL, from mouse

别名:

streptavidin binding peptide

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

20, monoclonal

种属反应性

human

浓度

1.0 mg/mL

技术

immunocytochemistry: suitable
western blot: suitable

同位素/亚型

IgG1κ

运输

wet ice

靶向翻译后修饰

unmodified

一般描述

链霉亲和素结合肽标签是一种有用且用途广泛的肽,可以插入表达载体中,以便于重组蛋白的检测和纯化。SBP标签的序列长38个氨基酸,对链霉亲和素具有纳摩尔规模结合亲和力,平衡常数为2.5 nM。

特异性

证明与表达SBP标签的细胞反应。
该抗体识别SBP标签。

免疫原

对应于SBP标签的线性肽。
表位:未知

应用

免疫荧光分析: 先前批次已被独立实验室用于IF。(Hudson, D., et al. (2008).MBC. 19(7):3070-3079.)
研究子类别
表位标签
研究类别
表位标签 & 一般用途

质量

已通过蛋白质印迹在pTrAP-GFP转染的Hek293裂解物中进行了评估。

蛋白质印迹分析:0.1 ng/ml的该抗体在10 µg pTrAP-GFP转染的Hek293裂解物上检测到SBP标签。

目标描述

蛋白表达是包含组氨酸标签,S标签,链霉亲和素结合肽(SBP)标签和GFP的pTrAP-GFP质粒。表达的蛋白约为37kDa

外形

在含 0.1 M Tris-甘氨酸(pH 7.4,150 mM NaCl) 和 0.05% 叠氮化钠的缓冲液中的纯化的小鼠单克隆 IgG1κ。
形式:纯化
纯化的蛋白G

储存及稳定性

自收到之日起,在 2-8°C 条件下可稳定保存1年

分析说明

对照
pTrAP-GFP转染的Hek293裂解物

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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